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Invitrogen™ SuperScript™ III Reverse Transcriptase
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Specifications:
| Application | Reverse Transcription | ||
| Storage Temperature | -20°C | ||
| Product Type | Molecular Biology Reagent | Forms | Kit with Various components |
| Product Brand | Thermo Fisher Scientific™, Invitrogen | ||
| Product Grade | Molecular Biology | ||
High-performance MMLV-derived RT for robust and efficient cDNA synthesis from diverse RNA templates
Invitrogen™ SuperScript™ III Reverse Transcriptase is a genetically engineered Moloney Murine Leukemia Virus (MMLV) reverse transcriptase with reduced RNase H activity, enhanced thermal stability, and improved half-life. Engineered through specific mutations in the pol gene, SuperScript III offers high-yield, full-length cDNA synthesis, even from GC-rich or difficult RNA templates.
Trusted by researchers with over 50,000 citations, this reverse transcriptase consistently outperforms wild-type MMLV and RNase H-minus variants in first-strand cDNA synthesis applications and is ideal for gene expression studies, RT-PCR, cloning, and sequencing.
Key Features
- High Temperature Capability: Synthesizes cDNA at temperatures up to 55°C, minimizing RNA secondary structure interference
- Long-Range Performance: Generates full-length cDNA products from 100 bp to >12 kb
- Enhanced Thermostability: Maintains activity across prolonged incubations
- Improved Specificity & Yields: Delivers higher cDNA yields with reduced nonspecific priming
- Low RNase H Activity: Preserves RNA template integrity during first-strand synthesis
- Purified to Near Homogeneity: Expressed in E. coli and stringently purified for research use
Applications
- First-strand cDNA synthesis for RT-PCR and qRT-PCR
- Cloning of full-length cDNAs
- Reverse transcription of GC-rich or structured RNA templates
- Transcriptome and gene expression analysis
- Primer extension assays
- Synthesis of cDNA libraries
Kit Contents (per 10 Reaction Pack)
| Component | Volume |
|---|---|
| SuperScript™ III RT (200 U/μL) | 10 µL (2,000 units) |
| 5X First-Strand Buffer | 1 mL |
| DTT (100 mM) | 500 µL |
Specifications
| Specification | Details |
|---|---|
| Catalog Numbers | 18080093 (10 rxn), 18080044 (50 rxn), 18080085 (200 rxn) |
| Final Product Type | First-strand cDNA |
| Enzyme Type | SuperScript™ III Reverse Transcriptase |
| Reaction Format | Stand-alone enzyme with separate components |
| Concentration | 200 U/μL |
| Units per Reaction | 200 Units |
| Number of Reactions | 10 / 50 / 200 (based on unit size) |
| Optimal Reaction Temp | 50–55°C |
| Template | Total RNA or poly(A)+ RNA |
| Size Range (cDNA) | 100 bp to >12.3 kb |
| RNase H Activity | Reduced |
| GC-Rich RNA Performance | High |
| Reaction Time | 30–50 minutes |
| Shipping Condition | Dry ice |
| Storage Condition | –20°C |
| Regulatory Status | Research Use Only (RUO) |
Invitrogen™ SuperScript™ III Reverse Transcriptase is the trusted solution for high-efficiency cDNA synthesis, offering superior thermal tolerance, longer product synthesis, and robust performance even with challenging RNA templates. Ideal for routine and advanced molecular biology workflows, this enzyme enables sensitive, specific, and reliable reverse transcription results across a wide range of applications.
- Reactions: 50 rxn 200 rxn 10 rxn