Invitrogen™ Qubit™ dsDNA Broad Range (BR) Assay Kit, 4 to 2000 ng

The Qubit™ dsDNA Broad Range (BR) Assay Kit is designed for accurate and selective quantification of double-stranded DNA (dsDNA) in a broad range of concentrations. It is ideal for routine DNA quantification, supporting 500 reactions per kit and a quantitation range of 4 to 2000 ng. This assay is compatible with all Qubit Fluorometers and is suitable for a wide variety of molecular biology applications.

Key Features

1. Broad Quantitation Range:
   • Measures dsDNA concentrations from 4 to 2000 ng.
   • Suitable for high-abundance DNA samples, such as plasmid or genomic DNA.
2. High Selectivity:
   • Distinguishes dsDNA from contaminants like:
     • Single-stranded DNA (ssDNA).
     • RNA, proteins, and free nucleotides.
3. Precision and Robustness:
   • Reliable quantification even in the presence of salts, solvents, or detergents.
   • Fluorescence-based detection ensures high sensitivity and accuracy.
4. Simple Workflow:
   • Concentrated assay reagent and pre-diluted DNA standards included.
   • Mix reagent with the provided dilution buffer, add 1–20 µL of your sample, and measure using a Qubit Fluorometer.
5. Flexible Applications:
   • Suitable for quantifying plasmid DNA, genomic DNA, or other high-abundance samples.
6. High Throughput:
   • Provides enough reagents for 500 reactions, making it ideal for labs with frequent DNA quantification needs.

Specifications

Kit Components

• Concentrated Assay Reagent: Mix with dilution buffer for fluorescence detection.
• Dilution Buffer: Provided to dilute the assay reagent.
• Pre-Diluted DNA Standards: Ensure accurate calibration.

Compatible Equipment

• Qubit Fluorometers:
  • Qubit 4 Fluorometer.
  • Qubit Flex Fluorometer.
  • Qubit 3.0 and 2.0 Fluorometers.
• Consumables:
  • Thin-wall, clear 0.5-mL PCR tubes (Cat. No. Q32856) for Qubit 4.
  • 8 x 200-µL tube strips (Cat. No. Q33252) for Qubit Flex.

Applications

1. Plasmid and Genomic DNA Quantification:
   • Ideal for routine DNA analysis.
2. Sequencing Preparation:
   • Accurate DNA input quantification for NGS library prep.
3. Molecular Biology Research:
   • Suitable for general DNA quantification in molecular workflows.
4. Quality Control:
   • Verify DNA extraction yield and purity.

Advantages

• Broad Range Coverage:
  • Supports low- to high-abundance DNA quantification needs.
• Ease of Use:
  • Simple and quick protocol, suitable for high-throughput workflows.
• High Selectivity:
  • Minimizes interference from contaminants, ensuring accurate results.

Storage and Shipping

• Shipping Condition: Room temperature.
• Storage: Store at room temperature for long-term stability.

Why Choose Qubit dsDNA BR Assay Kits?

• Highly selective for dsDNA over ssDNA, RNA, and proteins.
• Efficient for labs handling high-throughput or high-concentration DNA quantification needs.
• Compatible with all Qubit Fluorometer models for reliable results.

This kit is an ideal solution for researchers seeking accurate, high-throughput DNA quantification across a wide concentration range.