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Invitrogen™ PureLink™ Quick Plasmid Miniprep Kit
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Specifications:
| Application | DNA Extraction and Purification | ||
| Storage Temperature | 2-8°C | ||
| Product Type | Sample Prep | Forms | Kit with Various components |
| Product Brand | Thermo Fisher Scientific™, Invitrogen | ||
| Product Grade | Molecular Biology | ||
The Invitrogen™ PureLink™ Quick Plasmid Miniprep Kit provides a fast, reliable, and cost-effective method for isolating high-quality plasmid DNA from E. coli cultures. Using silica spin column technology, the kit delivers sequencing-grade DNA in as little as 30–45 minutes from 1–5 mL bacterial cultures.
The workflow is simple: bacterial cells are lysed by an alkaline/SDS procedure, plasmid DNA binds to the silica membrane, impurities are washed away, and pure DNA is eluted in TE buffer. Yields of up to 40 μg plasmid DNA are achieved, suitable for downstream molecular biology applications.
Key Features
- Fast Protocol – Ready-to-use plasmid DNA in 30–45 minutes.
- Flexible Formats – Compatible with both centrifuge and vacuum manifolds.
- High Yield – Recovers up to 40 μg plasmid DNA from 1–5 mL cultures.
- Sequencing-Grade Purity – Typical A260/A280 > 1.8, free of proteins and inhibitors.
- Reliable & Economical – Trusted Invitrogen quality with consistent results.
Applications
- Cloning and ligation workflows.
- DNA sequencing (manual and automated).
- PCR amplification and downstream enzymatic reactions.
- Bacterial and mammalian cell transformation.
- Restriction enzyme digestion.
Technical Specifications
| Parameter | Details |
|---|---|
| Product Type | Quick Plasmid Miniprep Kit |
| Catalog Numbers | K210010 (50 preps), K210011 (250 preps) |
| Isolation Technology | Silica spin column |
| Sample Type | E. coli bacterial culture |
| Sample Volume | 1–5 mL |
| Prep Scale | <100 μg (small-scale plasmid prep) |
| Yield | Up to 40 μg plasmid DNA |
| DNA Quality | A260/A280 > 1.8 |
| Test Time | 30–45 minutes |
| Concentration | RNase A (20 mg/mL) included |
| Applications | Sequencing, cloning, PCR, transformation, restriction digestion |
| High-Throughput | Manual use (not automation compatible) |
| Shipping Condition | Room temperature |
| Storage Condition | Store kit components as recommended (buffers and RNase at 2–8 °C) |
| Unit Size | Each kit (50 or 250 preps) |
Kit Contents
- Resuspension buffer
- RNase A (20 mg/mL)
- Lysis buffer
- Precipitation buffer
- Wash buffers
- TE buffer
- Spin columns in wash tubes
- Elution tubes
Unique Advantages
- Higher yield and purity compared to traditional miniprep kits.
- Flexible protocol with vacuum or centrifuge processing.
- Provides reliable sequencing-grade plasmid DNA every time.
- Convenient format suitable for both low- and medium-throughput laboratories.
The Invitrogen™ PureLink™ Quick Plasmid Miniprep Kit is an essential tool for laboratories requiring fast, high-yield plasmid DNA isolation. Its combination of silica spin column efficiency, sequencing-grade DNA purity, and flexible compatibility with vacuum or centrifuge systems makes it ideal for a wide range of molecular biology applications including cloning, sequencing, PCR, and transformation.
- Pack Size: 50 Preps 250 Preps