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Specifications:
| Application | PCR | ||
| Storage Temperature | Room Temperature | ||
| Product Type | PCR Reagent | Forms | Kit with Various components |
| Product Brand | Thermo Fisher Scientific™, Invitrogen | ||
| Product Grade | Molecular Biology | ||
The Invitrogen™ PureLink™ PCR Micro Kit is a high-efficiency DNA purification system optimized for low-yielding PCR or restriction digestion reactions. Using silica spin column technology, the kit purifies and concentrates DNA fragments ranging from 100 bp to 12 kb in as little as 10 minutes.
Its innovative micro-nozzle spin column design ensures up to 95% recovery, even when working with small samples and low elution volumes (as little as 5 μL). The kit efficiently removes >98.5% of primers, unincorporated dNTPs, salts, enzymes, and dye-labeled nucleotides, delivering DNA suitable for sensitive downstream molecular biology applications.
Key Features
- High Recovery – Up to 95% recovery of DNA fragments.
- Small Elution Volumes – Recover DNA in as little as 5 μL.
- Fast Protocol – Complete purification in ~10 minutes.
- Wide Range – Purifies DNA fragments between 100 bp and 12 kb.
- Efficient Removal – Eliminates primers, dNTPs, enzymes, salts, and dye-labeled nucleotides.
- Consistent Results – <10% CV (coefficient of variance) in elution volume ensures reproducibility.
Applications
- Purification and concentration of PCR amplicons.
- Clean-up of DNA from restriction digestion reactions.
- Removal of labeled nucleotides after cDNA labeling.
- Preparation of DNA for sequencing, cloning, PCR, and labeling.
Technical Specifications
| Parameter | Details |
|---|---|
| Product Type | PCR Micro Kit |
| Catalog Numbers | K310050 (50 preps), K310250 (250 preps) |
| Isolation Technology | Silica spin column |
| Sample Type | PCR products, restriction digests |
| Separation Range | 100 bp – 12 kb |
| Starting Material | ≤125 μL |
| Elution Volume | 5–20 μL |
| Yield | High recovery, up to 95% |
| Removal Efficiency | >98.5% of primers, dNTPs, salts, enzymes |
| Run Time | 10 minutes |
| High-Throughput | Manual use only |
| Shipping Condition | Room temperature |
| Storage | Room temperature |
| Unit Size | Each kit (50 or 250 preps) |
Kit Contents
- Silica spin columns with micro-nozzle design
- Binding buffer
- Wash buffer
- Recovery tubes
- User manual
Unique Advantages
- Optimized for low-yield reactions, ensuring no DNA is lost.
- Works with very small elution volumes for high-concentration DNA recovery.
- Provides sequencing-grade DNA suitable for all routine downstream applications.
- Faster, safer, and more consistent than traditional ethanol precipitation.
The Invitrogen™ PureLink™ PCR Micro Kit is the ideal choice for purifying and concentrating DNA from low-yield PCR or enzymatic reactions. With its micro-nozzle technology, fast 10-minute workflow, and high recovery rates, it provides consistent, high-quality DNA for sequencing, cloning, labeling, PCR, and other molecular biology applications.
- Pack Size: 50 Preps 250 Preps