0
Invitrogen™ ChargeSwitch™ PCR Clean-Up Kit
Catalog No :
CAS Number :
Brand :
In Stock
Specifications:
| Application | PCR | ||
| Storage Temperature | Room Temperature | ||
| Product Type | PCR Reagent | Forms | Liquid |
| Product Brand | Thermo Fisher Scientific™, Invitrogen | ||
| Product Grade | Molecular Biology | ||
The Invitrogen™ ChargeSwitch™ PCR Clean-Up Kit provides a fast, efficient, and scalable method for purifying PCR products by removing primers, unincorporated dNTPs, thermostable polymerases, and salts. Using ChargeSwitch™ magnetic bead technology, the kit enables high DNA recovery from 25–50 μL PCR reactions with a rapid, one-tube protocol completed in as little as 5 minutes.
This kit is available in 100-prep (CS12000) and 960-prep (CS1200010) formats, making it suitable for both standard and high-throughput applications, including use with liquid-handling robotic platforms. It is supplied with all necessary buffers and reagents, requiring only the use of a compatible magnetic accessory for DNA binding and recovery.
Key Features
- Fast Protocol – Complete purification in just 5 minutes.
- High Recovery – Optimized for maximum yield of DNA fragments from PCR reactions.
- Magnetic Bead-Based – One-tube purification reduces handling and sample loss.
- Scalable & Automated – Compatible with manual workflows or high-throughput robotics.
- Complete Kit – Includes all necessary reagents and buffers for reliable DNA clean-up.
- Flexible Applications – Ideal for downstream use in NGS, cloning, and PCR-based workflows.
Applications
- Removal of primers, dNTPs, polymerases, and salts from PCR reactions.
- Preparation of DNA for next-generation sequencing (NGS).
- PCR product clean-up prior to cloning and ligation workflows.
- Sample preparation for restriction digestion, labeling, or other enzymatic reactions.
- High-throughput DNA clean-up in automated systems.
Technical Specifications
| Parameter | Details |
|---|---|
| Product Type | PCR Clean-Up Kit |
| Catalog Numbers | CS12000 (100 preps), CS1200010 (960 preps) |
| Format | Kit with magnetic bead chemistry |
| Sample Size | 25–50 μL PCR reaction |
| Run Time | ~5 minutes |
| Target | DNA from enzymatic reactions (PCR) |
| Technology | ChargeSwitch™ Magnetic Beads |
| High-Throughput | Compatible with automation/robotics |
| Applications | NGS, cloning, PCR product clean-up |
| Quantity | 100 or 960 preps |
| Shipping Condition | Room temperature |
| Storage Conditions | Room temperature (stable for 6 months) |
Kit Components
- ChargeSwitch™ Magnetic Beads (25 mg/mL in MES buffer)
- Purification Buffer (N5)
- Wash Buffer (W12)
- Elution Buffer (E5; 10 mM Tris-HCl, pH 8.5)
Unique Advantages
- Eliminates the need for spin columns or centrifugation.
- Minimal hands-on time with simplified magnetic bead workflow.
- Compatible with downstream sensitive molecular biology applications.
- Scalable for both small-volume labs and high-throughput facilities.
The Invitrogen™ ChargeSwitch™ PCR Clean-Up Kit is a rapid, magnetic bead–based solution for purifying DNA from PCR reactions. With its fast 5-minute protocol, high recovery rates, and compatibility with both manual and automated workflows, it is a versatile and reliable reagent for DNA purification in cloning, sequencing, and molecular biology research.
- Pack Size: For 100 Preps For 960 Preps