Invitrogen™ MEGAscript™ T7 Transcription Kit

The Invitrogen™ MEGAscript™ T7 Transcription Kit is an ultra-high-yield in vitro transcription system designed for the efficient synthesis of large amounts of RNA. By employing optimized reaction conditions and a patented high-yield technology, the kit enables the use of very high nucleotide concentrations, resulting in 10–50 times more RNA compared to conventional transcription reactions.

Each lot of T7 Enzyme Mix and 10X Reaction Buffer is specifically calibrated for performance with T7 RNA polymerase, ensuring consistency, reliability, and reproducibility across RNA synthesis workflows.

Key Features

• Ultra-high yield technology – produces 3–5 mg of RNA per kit, approximately 100 µg or more per reaction from the control template.
• High efficiency – typically yields over 10 times more RNA than standard transcription systems.
• Flexible applications – suitable for unlabeled or low-specific-activity RNA, in vitro translation, antisense/microinjection studies, and RNA-binding protein isolation.
• Calibrated reagents – each lot of T7 enzyme mix and buffer optimized for maximum transcription efficiency.
• Patented process – ensures 10–50x higher output than conventional kits.
• Complete kit – includes nucleotides, buffers, DNase, precipitation solutions, control template, and loading buffer.

Applications

• In vitro transcription of synthetic mRNA.
• mRNA preparation for transfection and delivery (e.g., lipid nanoparticles).
• Antisense studies and microinjection experiments.
• Large-scale RNA production for in vitro translation assays.
• RNA-binding protein isolation studies.
• Compatible with PCR products and linearized DNA plasmid templates.

Technical Specifications

Available Configurations

Contents & Storage

Each kit includes:

• T7 Enzyme Mix
• 10X Reaction Buffer
• ATP, CTP, GTP, and UTP solutions
• pTRI-Xef control template
• TURBO DNase
• Ammonium Acetate Stop Solution
• Lithium Chloride Precipitation Solution
• Gel Loading Buffer II
• Nuclease-free water

All components must be stored at –20 °C, except nuclease-free water, which can be stored at any temperature.

The Invitrogen™ MEGAscript™ T7 Transcription Kit is the industry standard for large-scale in vitro RNA synthesis, delivering unmatched yield and consistency. Optimized for T7 promoter-driven transcription, it enables researchers to generate abundant RNA for a wide range of applications including synthetic mRNA preparation, transfection, translation, and RNA functional studies. With its complete set of reagents and high-performance enzyme system, it ensures efficiency, reproducibility, and confidence in every RNA synthesis experiment.