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Invitrogen™ eBioscience™ Foxp3 / Transcription Factor Staining Buffer Set
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Specifications:
| Application | Flow Cytometry | ||
| Storage Temperature | Room Temperature | ||
| Product Type | Molecular Biology Reagent | Forms | Liquid |
| Product Brand | Thermo Fisher Scientific™, Invitrogen | ||
| Product Grade | Molecular Biology | ||
Optimized Reagent System for Intracellular Detection of Transcription Factors and Nuclear Proteins
The Invitrogen™ eBioscience™ Foxp3 / Transcription Factor Staining Buffer Set is a complete, validated solution for intracellular staining of transcription factors and nuclear antigens such as Foxp3, Ki-67, and cytokines. This buffer system is specifically optimized for use with fluorochrome-conjugated antibodies in flow cytometric analysis, enabling accurate detection of low-abundance or intracellularly localized targets.
The kit includes all necessary buffers for fixation and permeabilization and is particularly effective for identifying regulatory T cells (Tregs) through Foxp3 expression.
Key Features and Benefits
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Complete Buffer Set for Intracellular Staining
Includes fixation and permeabilization reagents and diluents, eliminating the need for separate components. -
Optimized for Transcription Factors
Designed for sensitive detection of nuclear and intranuclear proteins such as Foxp3 and Ki-67. -
Compatible with Multicolor Flow Cytometry
Maintains fluorochrome integrity, including tandem dyes, for use in high-dimensional immune profiling. -
Validated in Treg and Proliferation Studies
Trusted for accurate detection of regulatory T cell markers, cell proliferation, and immune activation.
Applications
- Intracellular staining of transcription factors (e.g., Foxp3, Ki-67)
- Flow cytometric analysis of regulatory T cells (Tregs)
- Proliferation assays and cytokine expression analysis
- Immune cell subset characterization and functional studies
Technical Specifications
| Property | Details |
|---|---|
| Product Name | Foxp3 / Transcription Factor Staining Buffer Set |
| Catalog Number | 00-5523-00 |
| Reagent Type | Fixation and permeabilization reagents and diluents |
| Compatible Targets | Transcription factors, nuclear proteins, cytokines, chemokines |
| Application | Intracellular staining, flow cytometry |
| Unit Size | 1 kit |
| Product Line | eBioscience™ |
| Storage Conditions | Store according to label instructions; protect from light |
| Shipping Conditions | Ambient |
| Intended Use | For Research Use Only. Not for use in diagnostic procedures. |
Kit Components
- Fixation/Permeabilization Concentrate
- Fixation/Permeabilization Diluent
- Permeabilization Buffer (10X)
All components have been optimized for use together. For individual reagents, refer to:
- Fixation/Permeabilization Conc. + Diluent: Cat. No. 00-5521-00
- Permeabilization Buffer (10X): Cat. No. 00-8333-56
The Invitrogen™ eBioscience™ Foxp3 / Transcription Factor Staining Buffer Set is the gold standard for intracellular flow cytometry workflows, especially in Treg biology and immune regulation research. It provides a reliable, consistent protocol for staining nuclear targets while preserving fluorochrome brightness and cell viability.