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Invitrogen™ IL-10 Monoclonal Antibody (JES5-16E3), PerCP-Cyanine5.5, eBioscience™
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Specifications:
| Application | Flow Cytometry | ||
| Storage Temperature | 2-8°C | ||
| Product Type | Primary Antibodies | Forms | Liquid |
| Product Brand | Thermo Fisher Scientific™, Invitrogen | ||
| Product Grade | Molecular Biology | ||
Rat Anti-Mouse IL-10 | Intracellular Cytokine Detection Antibody for Flow Cytometry
The Invitrogen™ IL-10 Monoclonal Antibody (Clone JES5-16E3) conjugated to PerCP-Cyanine5.5 is a validated tool for intracellular flow cytometric detection of murine interleukin-10 (IL-10). IL-10 is a potent anti-inflammatory cytokine secreted by a variety of immune cell types including Th2 cells, macrophages, B1 cells, and keratinocytes.
This antibody is optimized for intracellular staining of cytokine-expressing cells and is compatible with blue-laser flow cytometers.
Key Features and Benefits
- Clone JES5-16E3: Highly specific monoclonal antibody against mouse IL-10 (18 kDa), also known as Cytokine Synthesis Inhibitory Factor (CSIF).
- PerCP-Cyanine5.5 Conjugation: Ideal for 488 nm excitation, with 695 nm emission, providing a bright and distinct signal for multiparameter panels.
- Validated for Flow Cytometry: Widely cited and tested for intracellular staining of cytokine-producing murine immune cells.
- Stable Liquid Format: Filtered and ready-to-use antibody solution with 0.2 μm sterile filtration.
Applications
- Flow Cytometry (Tested): ≤0.5 µg/test in 100 µL
- Intracellular Cytokine Staining (ICS)
- Immune profiling of regulatory T cells, macrophages, and Th2 responses
- IL-10 functional studies in inflammation, autoimmunity, and infection models
Optimal antibody concentration should be titrated for specific assay conditions.
Technical Specifications
| Property | Details |
|---|---|
| Clone | JES5-16E3 |
| Host / Isotype | Rat / IgG2b, kappa |
| Conjugate | PerCP-Cyanine5.5 |
| Excitation / Emission Max | 489 / 679 nm (Peak emission ~695 nm) |
| Species Reactivity | Mouse |
| Published Reactivity | Mouse, Human |
| Tested Applications | Flow Cytometry (Intracellular) |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 0.09% sodium azide |
| Storage Conditions | 4°C, protect from light, Do Not Freeze |
| Shipping Conditions | Ambient (domestic), Wet ice (international) |
| Recommended Isotype Control | Rat IgG2b kappa Isotype Control, PerCP-Cyanine5.5 (eB149/10H5) |
| RRID | AB_996677 |
| Filtration | 0.2 µm post-manufacturing filtered |
Available Pack Sizes
| Catalog Number | Quantity | Format |
|---|---|---|
| 45-7101-82 | 100 µg | Liquid |
Target Information – IL-10
Interleukin-10 (IL-10) is a cytokine with broad anti-inflammatory effects. It is mainly produced by monocytes, Th2 cells, and other immune cells. IL-10 plays critical roles in limiting immune responses and promoting immune tolerance by suppressing Th1 cytokine production, MHC class II expression, and co-stimulatory molecule activity on antigen-presenting cells.
IL-10 is also a key regulator of the JAK-STAT pathway and is involved in various disease states, including autoimmune diseases, HIV infection, and graft-versus-host disease.
Invitrogen™ IL-10 Monoclonal Antibody (Clone JES5-16E3), PerCP-Cyanine5.5 is a robust, publication-supported antibody for detecting IL-10 in murine immune cells via flow cytometry. Its high specificity and brightness make it ideal for multicolor immune profiling and functional cytokine studies in inflammation, infection, and immune regulation research.
- Pack Size: 25 µg 100 µg