Human Oxidized Low-Density Lipoprotein, OxLDL ELISA Kit

The Human OxLDL ELISA Kit is a high-sensitivity, sandwich-based ELISA designed for the quantitative measurement of oxidized low-density lipoprotein (OxLDL) in human serum, plasma, culture media, tissue homogenates, and other biological fluids. This assay is ideal for cardiovascular and lipid oxidation research, offering precision, reproducibility, and a detection limit as low as 3.8 pg/mL.

Key Features

• Assay Type: Sandwich ELISA
• Detection Range: 15 – 800 pg/mL
• Sensitivity: 3.8 pg/mL
• Sample Volume: 10 μL
• Storage: 2–8 °C
• Shelf Life: 6 months
• Assay Time: ~2.5 hours
• Precision:
  • Intra-assay CV <10%
  • Inter-assay CV <12%

Principle of the Assay

This ELISA utilizes a double-antibody sandwich technique. The pre-coated microplate contains an anti-human OxLDL capture antibody. After sample addition, HRP-conjugated detection antibody binds to the OxLDL-antibody complex. Upon substrate addition (TMB), color develops proportionally to the amount of OxLDL. The reaction is stopped, and absorbance is read at 450 nm, allowing quantification based on a standard curve.

Kit Contents

Sample Types & Preparation

Validated for:

• Serum & Plasma: Centrifuge at 2,000–3,000 rpm for 20 minutes.
• Cell Culture Supernatants: Centrifuge post-culture.
• Tissue Homogenates: Homogenize in PBS (pH 7.4) at 4 °C, centrifuge, and collect supernatant.
• Urine & Body Fluids: Same procedure as above.

Avoid sodium azide (NaN₃) and repeated freeze-thaw cycles. Samples can be stored at –20 °C if not analyzed immediately.

Assay Procedure Overview

1. Prepare standards (dilute from 1080 to 60 pg/mL).
2. Add 40 μL sample diluent + 10 μL sample to wells.
3. Incubate for 30 minutes at 37 °C.
4. Wash the plate 5 times with diluted wash buffer.
5. Add 50 μL HRP-conjugated antibody to each well.
6. Incubate again at 37 °C for 30 minutes.
7. Wash again.
8. Add 50 μL each of Chromogen A and B. Incubate 15 minutes at 37 °C in the dark.
9. Add 50 μL stop solution; measure absorbance at 450 nm within 15 minutes.

Data Analysis

• A standard curve is constructed using log-log plot of OD vs. concentration.
• Sample concentrations are extrapolated from the curve and multiplied by dilution factor.
• All standards and samples should be run in duplicate.

Precision and Performance

The Human OxLDL ELISA Kit (SL1319Hu) is a sensitive, specific, and easy-to-use assay system for detecting oxidized LDL, a key biomarker of oxidative stress and cardiovascular disease. With a low detection limit, high reproducibility, and compatibility across multiple sample types, this kit is a powerful tool for researchers investigating lipid peroxidation, atherosclerosis, inflammation, and metabolic syndromes. Whether you're working with serum, plasma, or cell culture media, this ELISA kit delivers accurate results to support your research objectives in lipid biochemistry and cardiovascular pathology.