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Human 8-Hydroxy-deoxyguanosine (8-OHdG) ELISA Kit
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Specifications:
| Application | ELISA-Based Assays | ||
| Storage Temperature | 2-8°C | ||
| Product Type | Elisa Kit | Forms | Kit with Various components |
| Product Grade | Molecular Biology | ||
The Human 8-Hydroxy-deoxyguanosine (8-OHdG) ELISA Kit is a sensitive and quantitative enzyme-linked immunosorbent assay designed for the measurement of 8-OHdG, a widely recognized biomarker of oxidative DNA damage. 8-OHdG is formed when reactive oxygen species (ROS) attack guanine bases in DNA, making it a critical indicator in studies of oxidative stress, aging, cancer, inflammation, neurodegeneration, cardiovascular diseases, and environmental toxicity.
This kit enables accurate quantification of human 8-OHdG levels in serum, plasma, urine, culture media, cell lysates, tissue homogenates, and other biological fluids, supporting a broad range of life-science and biomedical research applications.
Intended Use
This ELISA kit is intended for the quantitative detection of Human 8-OHdG antigen in biological samples for research purposes only.
Assay Principle
The assay is based on a solid-phase sandwich ELISA technique. Microplate wells are pre-coated with a high-affinity antibody specific for Human 8-OHdG. During the first incubation, 8-OHdG present in standards or samples binds to the immobilized antibody.
After washing to remove unbound materials, an HRP-conjugated secondary antibody specific to Human 8-OHdG is added, forming a stable antibody–antigen–enzyme complex. Excess conjugate is removed through additional wash steps.
The enzymatic reaction is initiated by the addition of TMB substrate, producing a blue color that is proportional to the amount of bound 8-OHdG. The reaction is stopped with an acidic stop solution, changing the color to yellow. Absorbance is measured at 450 nm using a microplate reader, and sample concentrations are calculated by comparison with a standard curve.
Key Features & Benefits
- High sensitivity with a detection limit of 16 pg/mL
- Wide assay range: 45 – 3000 pg/mL
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Excellent precision:
- Intra-assay CV < 10%
- Inter-assay CV < 12%
- Pre-coated microplate for improved consistency and ease of use
- Compatible with multiple sample types
- Quantitative and reproducible results
- Ready-to-use reagents minimize preparation time
Sample Compatibility
- Serum
- Plasma (EDTA or citrate)
- Urine
- Cell culture supernatants
- Cell lysates
- Tissue homogenates
- Other biological fluids
Kit Components
| Component | 96 Tests | 48 Tests | Storage |
|---|---|---|---|
| Instructions for Use | 1 | 1 | Room Temp |
| Adhesive Film/Membrane | 2 | 2 | Room Temp |
| Antibody-Coated Microplate | 1 | 1 | 2–8 °C |
| 8-OHdG Standard (3600 pg/mL) | 0.5 mL × 1 | 0.5 mL × 1 | 2–8 °C |
| Standard Diluent | 1.5 mL × 1 | 1.5 mL × 1 | 2–8 °C |
| HRP-Conjugate Reagent | 6 mL × 1 | 3 mL × 1 | 2–8 °C |
| Sample Diluent | 6 mL × 1 | 3 mL × 1 | 2–8 °C |
| Chromogen Solution A | 6 mL × 1 | 3 mL × 1 | 2–8 °C |
| Chromogen Solution B | 6 mL × 1 | 3 mL × 1 | 2–8 °C |
| Stop Solution | 6 mL × 1 | 3 mL × 1 | 2–8 °C |
| Wash Buffer Concentrate | 20 mL (30×) | 20 mL (20×) | 2–8 °C |
Materials Required (Not Provided)
- ELISA microplate reader (450 nm)
- 37 °C incubator
- Plate washer or manual washing system
- Adjustable micropipettes (10–1000 µL)
- Vortex mixer
- Distilled or deionized water
- Timer and absorbent paper
Performance Characteristics
| Parameter | Specification |
|---|---|
| Assay Type | Sandwich ELISA |
| Detection Wavelength | 450 nm |
| Sensitivity | 16 pg/mL |
| Assay Range | 45 – 3000 pg/mL |
| Precision (Intra-assay) | CV < 10% |
| Precision (Inter-assay) | CV < 12% |
Storage & Stability
Store the complete kit at 2–8 °C. Opened reagents remain stable until the stated expiry date when stored properly. Unused microplate strips should be resealed immediately with desiccant and used within 1–3 months.
The Human 8-OHdG ELISA Kit provides a reliable, sensitive, and quantitative solution for assessing oxidative DNA damage in a wide range of biological samples. Its robust performance, excellent reproducibility, and broad sample compatibility make it an essential tool for oxidative stress research, aging studies, cancer biology, toxicology, and biomarker discovery. Designed for ease of use and consistent results, this kit supports high-quality data generation in both academic and industrial research laboratories.
- Pack Size: For 48 Tests For 96 Tests