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Invitrogen™ 8-OHdG Competitive ELISA Kit, 96 Tests
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Specifications:
| Application | ELISA-Based Assays | ||
| Storage Temperature | 2-8°C | ||
| Product Type | Elisa Kit | Forms | Kit with Various components |
| Product Brand | Thermo Fisher Scientific™, Invitrogen | ||
| Product Grade | Molecular Biology | ||
The Invitrogen™ 8-OHdG Competitive ELISA Kit is a multispecies immunoassay designed for the quantitative measurement of 8-Hydroxy-2'-deoxyguanosine (8-OHdG) in serum, plasma, and other biological fluids. 8-OHdG is a key biomarker of oxidative DNA damage and mutagenesis, widely studied in cancer, aging, and oxidative stress research.
This competitive ELISA format delivers sensitivity and reproducibility, enabling researchers to accurately quantify oxidative stress-related DNA lesions across multiple sample types. Each lot is rigorously validated for sensitivity, precision, specificity, and consistency.
Key Features
- Competitive ELISA format – species-independent quantitative measurement of 8-OHdG.
- High sensitivity – detection as low as 0.94 ng/mL.
- Wide dynamic range – assay range 1.56–100 ng/mL.
- Reproducible performance – intra- and interassay CVs <10%.
- Validated kit – rigorous lot-to-lot testing for accuracy and reliability.
- Convenient format – complete 96-test kit with all essential reagents included.
Applications
- Measurement of oxidative stress biomarkers in biological fluids.
- Research on cancer, mutagenesis, and carcinogenesis.
- Studies of aging, neurodegeneration, and chronic disease.
- Assessment of oxidative DNA damage in serum, plasma, and urine samples.
- Evaluation of cellular responses to ROS and DNA repair pathways.
Technical Specifications
| Property | Value |
|---|---|
| Kit Format | Competitive ELISA |
| Catalog Number | EEL004 |
| Plate Format | 96 wells (pre-coated) |
| Sample Types | Serum, plasma, urine, biological fluids |
| Sample Volume | 50 µL |
| Analytical Sensitivity | 0.94 ng/mL |
| Assay Range | 1.56–100 ng/mL |
| Intraassay CV | <10% |
| Interassay CV | <10% |
| Hands-on Time | ~1 hr 20 min |
| Time-to-Result | ~2 hr 30 min |
| Detection Method | Absorbance (HRP/TMB) |
| Detector Antibody | Biotinylated, with HRP-conjugated avidin |
| Shipping Conditions | Wet ice |
| Storage Conditions | Refer to kit manual (component-specific) |
Kit Contents
- Pre-coated 96-well plate
- 8-OHdG standard
- Biotinylated detection antibody
- HRP conjugate
- Standard & sample diluent
- Antibody & conjugate diluents
- Wash buffer
- TMB substrate
- Stop solution
- Plate sealer
Target Information
- Biomarker: 8-Hydroxy-2'-deoxyguanosine (8-OHdG)
- Biological relevance: Major marker of oxidative DNA damage caused by reactive oxygen species (ROS).
- Pathophysiology: Associated with mutagenesis, GC→TA transversions, carcinogenesis, and aging.
- Excretion: Repair products of DNA (including 8-OHdG) are excreted in urine without further metabolism, making it a valuable non-invasive biomarker of oxidative stress.
The Invitrogen™ 8-OHdG Competitive ELISA Kit provides researchers with a robust, sensitive, and validated platform for quantifying oxidative DNA lesions. With its broad species compatibility, high reproducibility, and complete kit format, it is an essential tool for oxidative stress, DNA damage, mutagenesis, and cancer research.