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Specifications:
| Application | qPCR | ||
| Storage Temperature | -20°C | ||
| Product Type | PCR Reagent | Forms | Liquid |
| Product Brand | Genekam Biotechnology AG | ||
| Product Grade | Molecular Biology | ||
Comprehensive real-time PCR assay for HBV genotypes A, B, C, D, E, F, G, and H
The Genekam HBV Genotyping A–H Real-Time PCR Kit is a ready-to-use, in vitro research assay designed to detect and differentiate eight hepatitis B virus (HBV) genotypes (A through H) from DNA extracted from a variety of clinical and research samples.
This kit separates detection into two parts:
- Part 1 (Tube A) – Detects genotypes A, B, C, and D
- Part 2 (Tube C) – Detects genotypes E, F, G, and H
Each reaction uses a probe-based real-time PCR format with specific fluorogenic dyes for precise genotype identification. The assay is compatible with real-time PCR instruments equipped with at least four detection channels.
Key Features & Benefits
- Full HBV Genotype Coverage – Detects all eight known HBV genotypes (A–H) in two parallel reactions.
- Probe-Based Detection – High specificity with genotype-specific fluorescent probes.
- Multiplex Capability – Requires real-time PCR instruments with ≥4 channels for simultaneous detection.
- Broad Sample Compatibility – Works with DNA isolated from blood, serum, plasma, tissue, vaginal swabs, and other body fluids.
- Ready-to-Use Format – Includes all necessary reagents, positive and negative controls.
- Clear Result Interpretation – Ct values ≤40 indicate positive detection; 40–45 indicate marginal positive (doubtful).
Fluorophore Assignments
Part 1 – Tube A (A–D):
- FAM – HBV genotype A
- HEX/VIC – HBV genotype B
- ROX – HBV genotype C
- Cy5 – HBV genotype D
Part 2 – Tube C (E–H):
- FAM – HBV genotype E
- HEX/VIC – HBV genotype F and H
- ROX – HBV genotype G
Applications
- Research studies on HBV genotype distribution
- Epidemiological and molecular virology research
- Viral evolution and phylogenetic studies
- Laboratory validation of HBV molecular detection protocols
Technical Specifications
| Parameter | Details |
|---|---|
| Product Type | Real-time PCR kit for HBV genotyping |
| Genotypes Detected | A, B, C, D, E, F, G, H |
| Format | Ready-to-use reagents |
| No. of Tests | 100 |
| Kit Components | Tube A (2×), Tube B (2×), Tube C (2×), Positive Control (D1), Negative Control (D2) |
| Sample Types | Blood, serum, plasma, tissue, vaginal swabs, other body fluids |
| Detection Method | Fluorogenic probe-based real-time PCR |
| Required Equipment | Real-time PCR thermocycler with ≥4 detection channels |
| Shipping Condition | On dry ice |
| Storage Temperature | −20°C, protect from light |
| Shelf Life | 1 year from manufacture |
| Reference Number | FR193 |
| Unit Size | 100 tests |
The Genekam Biotechnology HBV Genotyping A–H Real-Time PCR Kit is a powerful tool for researchers studying the epidemiology and molecular diversity of hepatitis B virus. Its ability to accurately detect all eight genotypes using a two-tube, probe-based system ensures high specificity and reliable results across a range of sample types. With ready-to-use reagents, built-in controls, and compatibility with standard real-time PCR platforms, this kit is an essential resource for HBV genotyping in research laboratories.