0
Specifications:
| Application | Next Generation Sequencing, NGS Library Preparation | ||
| Storage Temperature | -20°C | ||
| Product Type | Sequencing Buffer, NGS library Prep kits | Forms | Liquid |
| Product Brand | Hologic Diagenode | ||
| Product Grade | Molecular Biology | ||
Diagenode Tagmentation Buffer (2x) is the recommended reagent to perform any tagmentation reactions. It can be used in combination with Diagenode Tagmentase (Tn5 transposase) on DNA or chromatin samples, as half of the total volume reaction like in ATAC-seq protocol.
ATAC-seq experiments:
- After cell lysis and nuclei isolation, the nuclei pellets can be incubated with the following mix for 1 reaction:
| Tagmentation Buffer (2x) | 25 µl |
| Tagmentase loaded | 2.5 µl |
| Digitonin 1% | 0.5 µl |
| Tween20 10% | 0.5 µl |
| PBS | 16.5 µl |
| Nuclease-free water | 5 µl |
| Nuclei pellet* |
* The number of nuclei per reaction will depend on the ATAC-seq experimental design. Successful tagmentation with the proposed protocol has been performed on 50,000 nuclei per reaction.
- The reaction is then incubated 30 minutes at 37°C.
- The tagmentation reaction can then be stopped by addition of 250 µl of DNA Binding buffer from Diagenode MicroChIP DiaPure Columns (Cat. No. C03040001).
- The tagmented libraries can then be purified using the MicroChIP DiaPure Columns (Cat. No. C03040001), and amplified.
- Pack Size: 100 μL 300 μl 1000 µl 5000 µl 10000 µl
Accessory Products
Add a Review
Customer Review
Showing
0
Of
0