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Specifications:
| Application | Next Generation Sequencing, NGS Library Preparation |
| Storage Temperature | -20°C |
| Product Type | NGS library Prep kits |
| Product Brand | Hologic Diagenode |
| Product Grade | Molecular Biology |
The Hologic Diagenode 24 UDI for Tagmented Libraries – Set III (C01011037) is a high-quality unique dual-index (UDI) primer set designed for multiplexed next-generation sequencing (NGS) on Illumina platforms. These UDI primers enable accurate and efficient sample barcoding, allowing for the simultaneous sequencing of up to 24 samples in a single run while minimizing index hopping and enhancing data integrity.
This UDI set is specifically validated for tagmentation-based library preparation protocols, ensuring compatibility with various chromatin profiling and sequencing applications such as ChIPmentation, ATAC-seq, and CUT&Tag technologies. Additionally, when combined with Set I (C01011034) and Set II (C01011036), up to 72 samples can be multiplexed in a single sequencing workflow, optimizing sequencing throughput and cost efficiency.
Key Features & Benefits
1. High-Throughput Sample Multiplexing
- Allows multiplexing of up to 24 samples per sequencing run on Illumina platforms.
- All three UDI sets (Set I, Set II, and Set III) can be combined, supporting up to 72-sample multiplexing.
2. Accurate & Reliable Indexing
- Unique dual-indexing (UDI) strategy prevents index hopping and misassignment of sequencing reads.
- Enables high-precision data analysis for sensitive epigenetics and transcriptomics applications.
3. Optimized for Tagmentation-Based Workflows
- Validated for use with ChIPmentation, ATAC-seq, and CUT&Tag library preparation kits, including:
- μChIPmentation for Histones (Cat. No. C01011011)
- ChIPmentation Kit for Histones (Cat. No. C01011009)
- TAG Kit for ChIPmentation (Cat. No. C01011030)
- ATAC-seq Kit (Cat. No. C01080002)
- Ensures efficient and high-quality sequencing results across a range of NGS applications.
Specifications
| Property | Details |
|---|---|
| Brand | Hologic Diagenode |
| Product Name | 24 UDI for Tagmented Libraries – Set III |
| Catalog Number | C01011037 |
| Indexing Type | Unique Dual Index (UDI) |
| Multiplexing Capacity | Up to 24 samples (expandable to 72 with Set I & Set II) |
| Compatibility | Tagmentation-based library preparation for Illumina sequencing |
| Application | ChIPmentation, ATAC-seq, CUT&Tag, NGS library preparation |
Use Cases & Applications
1. Epigenetics & ChIP Sequencing
- Ideal for ChIPmentation-based histone modification profiling.
- Enhances multiplexing efficiency in chromatin immunoprecipitation (ChIP) workflows.
2. ATAC-seq & Chromatin Accessibility Studies
- Enables accurate indexing in ATAC-seq library preparation.
- Provides high-fidelity dual indexing for chromatin accessibility sequencing.
3. CUT&Tag & Single-Cell Epigenomics
- Supports tagmentation-based CUT&Tag workflows for chromatin profiling.
- Allows for precise barcoding of single-cell epigenetics experiments.
4. Transcriptomics & Gene Expression Analysis
- Optimized for multiplexing RNA-seq and gene expression studies.
- Ensures accurate data analysis with minimal read misassignment.
Ordering Information
| Catalog Number | Description | Multiplexing Capacity |
|---|---|---|
| C01011037 | Hologic Diagenode 24 UDI for Tagmented Libraries – Set III | Up to 24 samples |
| C01011034 | Hologic Diagenode 24 UDI for Tagmented Libraries – Set I | Up to 24 samples |
| C01011036 | Hologic Diagenode 24 UDI for Tagmented Libraries – Set II | Up to 24 samples |
Note: All three sets can be combined for multiplexing up to 72 samples in a single sequencing run.
The Hologic Diagenode 24 UDI for Tagmented Libraries – Set III (C01011037) provides unique dual-index primers for accurate, high-throughput multiplexing of up to 24 samples in tagmentation-based sequencing workflows. Designed for Illumina sequencing platforms, this high-quality UDI set minimizes index hopping, ensuring reliable and reproducible sequencing results for ChIPmentation, ATAC-seq, and CUT&Tag applications.