Amersham™ Hybond-N+ Blotting membranes

Amersham™ Hybond-N⁺ is a high-sensitivity, positively charged nylon membrane designed specifically for nucleic acid blotting applications, including Southern, Northern, and array-based hybridization. With a nucleic acid binding capacity up to 600 µg/cm², Hybond-N⁺ offers superior performance in radioactive and non-radioactive detection methods, delivering high signal intensity and strong retention of target molecules.

The membrane’s positive charge enhances nucleic acid binding through electrostatic interaction, while its high tensile strength and chemical durability support multiple rounds of hybridization and stripping, making it ideal for reprobing experiments. UV crosslinking is recommended for optimal fixation and signal reproducibility. Baking is also possible, although less consistent. Hybond-N⁺ is not suitable for alkali blotting or Western blotting.

Key Features & Benefits

• High Binding Capacity
  Binds up to 600 µg/cm² of DNA or RNA for high sensitivity.
• Excellent Signal Retention
  Recommended for both radioactive and non-radioactive detection (chemiluminescence, chemifluorescence).
• Robust Membrane for Multiple Uses
  Strong mechanical stability enables multiple hybridizations and washes.
• Flexible Detection Compatibility
  Compatible with a wide range of detection reagents and hybridization protocols.
• Uniform Double-Sided Coating
  Both sides of the membrane are identical, eliminating orientation errors.
• Quality Assured
  Manufactured under strict QC for consistent results in life science research.

Technical Specifications

Applications

• Southern Blotting – DNA transfer and detection
• Northern Blotting – RNA analysis and transcript profiling
• cDNA or Oligo Arrays – High-density nucleic acid hybridizations
• Gene Expression Studies – mRNA and transcript validation
• Genomic Library Screening – Probe-based DNA identification

Available Product Variants

Amersham™ Hybond-N⁺ provides a reliable, high-capacity membrane solution for researchers conducting nucleic acid hybridization experiments. Whether working with radioactive probes, chemiluminescence systems, or fluorescent detection methods, Hybond-N⁺ delivers consistent performance, exceptional binding strength, and reusability in molecular biology workflows. Its wide range of available formats makes it adaptable to any laboratory setting, from high-throughput screening to small-scale gene expression studies.