Invitrogen™ EVOS™ Onstage Incubator (OSI-2)
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Specifications:
Application | Cell culture, Cell Analysis |
Storage Temperature | Ambient |
Product Type | Laboratory Equipment |
Product Brand | Thermo Fisher Scientific™, Invitrogen |
Product Grade | Molecular Biology |
The Invitrogen™ EVOS™ Onstage Incubator (OSI-2) is a second-generation, fully integrated environmental control system for live-cell, long-term, time-lapse imaging on the EVOS™ M5000 and EVOS™ M7000 Imaging Systems. It provides precise control of temperature, humidity, and gas composition (O₂, CO₂, N₂), enabling extended imaging sessions under physiological or hypoxic conditions without compromising cell health or experimental accuracy.
Designed for maximum convenience, the OSI-2 features an on-board air compressor, eliminating the need for an external air tank, and integrates seamlessly with EVOS software for direct control of environmental parameters and image acquisition settings.
Key Features & Benefits
- Complete Environmental Control – Maintain precise temperature, humidity, and gas composition for the entire duration of live-cell imaging experiments.
- Seamless Software Integration – Fully operated through the EVOS M5000 and M7000 user interface with automatic hardware recognition.
- On-Board Air Compressor – Eliminates the need for separate air tanks, simplifying setup.
- Flexible Vessel Compatibility – Supports slides, chambered slides, coverglass, multiwell plates, petri dishes, and T-25/T-75 flasks.
- Hypoxia Capability – Three gas line ports enable nitrogen purging for controlled low-oxygen environments.
- Quiet Operation – 45 dB with cover (48 dB without), ideal for sensitive laboratory environments.
- Stable Performance – ±0.1°C temperature stability minimizes thermal artifacts and focus drift.
Operating Ranges
Parameter | Range |
---|---|
Temperature | Ambient to 40°C (±0.1°C) |
Relative Humidity | 70–90% at 37–40°C |
CO₂ | 0–20% |
O₂ | 0% to ambient |
Noise Level | 45 dB (with cover), 48 dB (without cover) |
Specifications
Parameter | Control Unit | Environmental Unit |
---|---|---|
Dimensions (W x D x H) | 21 × 45 × 23 cm (8.3 × 18 × 9 in.) | 23 × 21 × 4.1 cm (9 × 11 × 1.6 in.) |
Weight | 6.7 kg (15 lb.) | 0.73 kg (1.6 lb.) |
Voltage | 100–240 V | — |
Frequency | 50/60 Hz | — |
Material Compatibility | — | Slides, chambered slides, chambered coverglass, multiwell plates, petri dishes, T-25 and T-75 flasks |
Contents & Accessories
- Stagetop environmental chamber
- Control unit
- Master stage plate (AMEPVH106)
- Light shield & cover
- Sensor data cable with 6-pin connector
- USB 2.0 A-to-B cable (180 cm)
- Heated hose with temperature control (180 cm; AMEP4728)
- Gas line (1/8 in. ID, 1/4 in. OD; AMEP4732)
- Push-to-connect gas line adaptors (1/4 in. NPT male, 3 each)
- Standard-head open-end wrench
- 2 mm hex screwdriver
- Power cord
Applications
- Long-term cell proliferation and viability studies
- Cell cycle and differentiation research
- Cell migration and wound-healing assays
- Organelle and membrane dynamics observation
- Hypoxic condition studies using nitrogen purging
The Invitrogen™ EVOS™ OSI-2 Onstage Incubator transforms the EVOS M5000 and M7000 into fully capable live-cell imaging platforms with stable, reproducible environmental control. Ideal for demanding time-lapse experiments, the OSI-2 ensures cells remain in optimal conditions for high-quality, reliable imaging data over hours or days.