Invitrogen™ EVOS™ Onstage Incubator (OSI-2)

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    Specifications:
    Application Cell culture, Cell Analysis
    Storage Temperature Ambient
    Product Type Laboratory Equipment
    Product Brand Thermo Fisher Scientific™, Invitrogen
    Product Grade Molecular Biology

    The Invitrogen™ EVOS™ Onstage Incubator (OSI-2) is a second-generation, fully integrated environmental control system for live-cell, long-term, time-lapse imaging on the EVOS™ M5000 and EVOS™ M7000 Imaging Systems. It provides precise control of temperature, humidity, and gas composition (O₂, CO₂, N₂), enabling extended imaging sessions under physiological or hypoxic conditions without compromising cell health or experimental accuracy.

    Designed for maximum convenience, the OSI-2 features an on-board air compressor, eliminating the need for an external air tank, and integrates seamlessly with EVOS software for direct control of environmental parameters and image acquisition settings.

    Key Features & Benefits

    • Complete Environmental Control – Maintain precise temperature, humidity, and gas composition for the entire duration of live-cell imaging experiments.
    • Seamless Software Integration – Fully operated through the EVOS M5000 and M7000 user interface with automatic hardware recognition.
    • On-Board Air Compressor – Eliminates the need for separate air tanks, simplifying setup.
    • Flexible Vessel Compatibility – Supports slides, chambered slides, coverglass, multiwell plates, petri dishes, and T-25/T-75 flasks.
    • Hypoxia Capability – Three gas line ports enable nitrogen purging for controlled low-oxygen environments.
    • Quiet Operation – 45 dB with cover (48 dB without), ideal for sensitive laboratory environments.
    • Stable Performance – ±0.1°C temperature stability minimizes thermal artifacts and focus drift.

    Operating Ranges

    ParameterRange
    TemperatureAmbient to 40°C (±0.1°C)
    Relative Humidity70–90% at 37–40°C
    CO₂0–20%
    O₂0% to ambient
    Noise Level45 dB (with cover), 48 dB (without cover)

    Specifications

    ParameterControl UnitEnvironmental Unit
    Dimensions (W x D x H)21 × 45 × 23 cm (8.3 × 18 × 9 in.)23 × 21 × 4.1 cm (9 × 11 × 1.6 in.)
    Weight6.7 kg (15 lb.)0.73 kg (1.6 lb.)
    Voltage100–240 V
    Frequency50/60 Hz
    Material CompatibilitySlides, chambered slides, chambered coverglass, multiwell plates, petri dishes, T-25 and T-75 flasks

    Contents & Accessories

    • Stagetop environmental chamber
    • Control unit
    • Master stage plate (AMEPVH106)
    • Light shield & cover
    • Sensor data cable with 6-pin connector
    • USB 2.0 A-to-B cable (180 cm)
    • Heated hose with temperature control (180 cm; AMEP4728)
    • Gas line (1/8 in. ID, 1/4 in. OD; AMEP4732)
    • Push-to-connect gas line adaptors (1/4 in. NPT male, 3 each)
    • Standard-head open-end wrench
    • 2 mm hex screwdriver
    • Power cord

    Applications

    • Long-term cell proliferation and viability studies
    • Cell cycle and differentiation research
    • Cell migration and wound-healing assays
    • Organelle and membrane dynamics observation
    • Hypoxic condition studies using nitrogen purging


    The Invitrogen™ EVOS™ OSI-2 Onstage Incubator transforms the EVOS M5000 and M7000 into fully capable live-cell imaging platforms with stable, reproducible environmental control. Ideal for demanding time-lapse experiments, the OSI-2 ensures cells remain in optimal conditions for high-quality, reliable imaging data over hours or days.

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