Applied Biosystems™ HIV-1 Genotyping Kit with Integrase

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    Specifications:
    Application PCR, Sanger Sequencing
    Storage Temperature -15°C to -25°C
    Product Type Sequencing Reagents Forms Kit with Various components
    Product Brand Thermo Fisher Scientific™, Applied Biosystems™
    Product Grade Molecular Biology

    Comprehensive Sanger Sequencing-Based Genotyping of HIV-1 Protease, Reverse Transcriptase, and Integrase Regions

    Catalog Number: A55120 | Quantity: 48 Tests | Format: Complete RT-PCR + Sequencing Kit

    Sample Types: HIV-1 RNA from EDTA plasma or dried blood spots (DBS)

    Shipping Condition: Dry Ice | Storage: –15°C to –25°C

    The Applied Biosystems™ HIV-1 Genotyping Kit with Integrase is a research-use molecular assay designed to detect and characterize drug resistance mutations (DRMs) within the protease (PR), reverse transcriptase (RT), and integrase (IN) regions of the HIV-1 pol gene. Covering codons 6–99 (PR), 1–251 (RT), and 1–288 (IN), the kit enables sequencing-based identification of resistance mutations associated with protease inhibitors (PI), nucleoside and non-nucleoside reverse transcriptase inhibitors (NRTI, NNRTI), and integrase strand transfer inhibitors (INSTI).

    Key Features and Benefits

    • Comprehensive Coverage – Simultaneous genotyping of PR, RT, and IN regions of the HIV-1 pol gene
    • Subtype Coverage – Detects HIV-1 subtypes A, B, C, D, F, G, CRF01_AE, CRF02_AG, and CRF06_cpx
    • Gold-Standard Accuracy – Sanger sequencing ensures high sensitivity and reproducibility
    • Complete Workflow Solution – RT-PCR, nested PCR, and bidirectional cycle sequencing in a single kit
    • Includes Internal Controls – Positive and negative RNA controls included
    • Flexible Sample Input – Use RNA extracted from EDTA plasma or dried blood spots (DBS)
    • CDC-Licensed Assays – Designed using validated HIV-1 genotyping methods from the U.S. CDC

    Kit Components

    • RNA Positive Control, 2 x 100 μL
    • HIV RNA Negative Control, 2 x 100 μL
    • RT-PCR Master Mix, PR/RT, 2 x 1.055 mL
    • RT-PCR Master Mix, IN, 2 x 1.055 mL
    • Nested-PCR Master Mix, PR/RT, 2 x 1.285 mL
    • Nested-PCR Master Mix, IN, 2 x 1.285 mL
    • SuperScript III One-Step RT-PCR with Platinum Taq High Fidelity Enzyme, 2 x 54 μL
    • AmpliTaq Gold LD DNA Polymerase, 1 x 54 μL
    • Sequencing vector, 2 x 80 μL
    • HIV Sequencing Mix R12, 2 x 435 μL
    • HIV Sequencing Mix R11, 2 x 435 μL
    • HIV Sequencing Mix F12, 2 x 435 μL
    • HIV Sequencing Mix F11, 2 x 435 μL
    • HIV Sequencing Mix R3, 2 x 435 μL
    • HIV Sequencing Mix R2, 2 x 435 μL
    • HIV Sequencing Mix R1, 2 x 435 μL
    • HIV Sequencing Mix F3, 2 x 435 μL
    • HIV Sequencing Mix F2, 2 x 435 μL
    • HIV Sequencing Mix F1, 2 x 435 μL

    Storage Conditions: –15°C to –25°C

    Technical Specifications

    AttributeDetails
    Target RegionsHIV-1 PR, RT, IN (pol gene)
    Genomic CoverageCodons 6–99 (PR), 1–251 (RT), 1–288 (IN)
    Detection MethodSanger Sequencing
    Enzymes IncludedSuperScript III, AmpliTaq Gold, Taq High Fidelity
    FormatRT-PCR, Nested PCR, Cycle Sequencing
    No. of Reactions48
    Sample InputRNA from EDTA plasma or dried blood spots
    Compatible InstrumentsVeriti™, GeneAmp™ 9700, ProFlex™, 3130/3500/SeqStudio Flex Genetic Analyzers
    Intended UseResearch only (RUO)

    Applications

    • Genotyping of HIV-1 for antiretroviral drug resistance research
    • Identification of mutations associated with PI, NRTI, NNRTI, and INSTI resistance
    • HIV molecular epidemiology studies
    • Retrospective clinical and surveillance research
    • Viral evolution and resistance mechanism analysis


    The Applied Biosystems™ HIV-1 Genotyping Kit with Integrase provides a complete, validated solution for the comprehensive genotyping of key resistance-related regions of the HIV-1 genome. Optimized for use with Sanger sequencing, it ensures accuracy and reproducibility across HIV-1 subtypes, supporting global research into drug resistance monitoring and viral evolution.

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