Applied Biosystems™ QuantStudio™ 5 Real-Time PCR System, 96-well, 0.2 mL, laptop

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Format: 96-well Plate
Block Sample Volume: 0.2mL

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Specifications:

Application	qPCR, PCR	Warranty	1 Year
Storage Temperature	Room Temperature
Product Type	thermal Cycler
Product Brand	Thermo Fisher Scientific™
Product Grade	Molecular Biology

Overview
Resource

The QuantStudio™ 5 Real-Time PCR System is designed for laboratories that demand superior performance, flexibility, and security. It delivers high sensitivity, broad multiplexing capabilities, and compliance with 21 CFR Part 11 guidelines, making it suitable for a wide range of applications, from basic research to regulated environments. Enhanced by OptiFlex technology and six independent VeriFlex zones, this system ensures reliable and precise results for genomic applications.

Key Features and Benefits

Advanced OptiFlex Technology:
- Features six decoupled excitation and emission filter channels and 21 filter combinations.
- Provides exceptional accuracy and sensitivity for multiplexing and single-plex reactions.
VeriFlex Temperature Control:
- Six independent temperature zones for precise PCR optimization and flexible protocol development.
21 CFR Part 11 Compliance:
- Built-in security features, including user account management, locked workflows, and audit trails.
- Supports compliance for regulated environments.
Connectivity with iConnect:
- Enables secure data upload, storage, and sharing from anywhere with cloud-based access.
- Streamlined tools for analysis through browser-based software modules.
Interactive Touchscreen Interface:
- Simplified instrument software for intuitive setup and operation.
- Pre-optimized protocol templates to reduce setup time.
Design and Analysis Software Options:
- Cloud-Based Software:
  - No installation required, accessible via PC or MAC.
  - Enables real-time data access and management.
- Desktop Software:
  - Traditional localized software for standalone use.
  - Compatible with Windows 10 OS.
Compact and User-Friendly Design:
- Factory-calibrated and delivered ready to use.
- Compact footprint saves valuable bench space.
High Multiplexing Capability:
- Supports up to 6 targets in a single run with its six optical channels.

Technical Specifications

Feature	Details
Block Configuration	96-well, 0.2 mL (also available in 0.1 mL)
Temperature Zones	6 independent VeriFlex zones
Dynamic Range	10 logs
Sensitivity	Detects 1.5-fold changes in target quantity
Optical Channels	6 decoupled channels, 21 filter combinations
Light Source	White LED
Software Options	Cloud-based or desktop Design and Analysis
Connectivity	USB, Ethernet, Wi-Fi
Dimensions	Compact design for bench space optimization
Calibration	Factory-calibrated
Applications	Gene expression, SNP genotyping, pathogen detection, HRM
Compliance	21 CFR Part 11 support

Applications

Gene Expression Analysis: Quantify mRNA levels with high precision.
Genetic Variation Studies: SNP genotyping and allele discrimination.
Pathogen Detection: Identify and quantify pathogen DNA or RNA in samples.
MicroRNA Profiling: Analyze small non-coding RNA molecules.
Epigenetics: Conduct high-resolution melting (HRM) analysis for methylation studies.

Key Advantages

Superior Multiplexing: Detect up to 6 targets simultaneously.
Regulatory Compliance: Built-in features for secure data handling and regulatory adherence.
Flexible Connectivity: Access and manage data from anywhere with cloud-enabled iConnect.
Compact and Reliable: Space-efficient design with robust factory calibration.
Broad Versatility: Optimized protocols for a wide array of genomic applications.

The Applied Biosystems™ QuantStudio™ series offers a range of real-time PCR systems tailored to various laboratory needs. Below is a comparative overview of the QuantStudio 3, 5, 6, and 7 models, highlighting their key features and differences.

Comparison Table: QuantStudio Real-Time PCR Systems and Others

Feature/Model	QuantStudio 3	QuantStudio 5	QuantStudio 6 Pro	QuantStudio 7 Pro	Bio-Rad CFX96 Touch	Roche LightCycler 480 II	Agilent AriaMx
Format	96-well, 0.1/0.2 mL	96-well, 0.1/0.2 mL; 384-well	Interchangeable: 96-well, 384-well, TaqMan Array	Interchangeable: 96-well, 384-well, TaqMan Array	96-well	96- or 384-well	96-well
Optical Channels	4 coupled	6 decoupled (21 filters)	5 colors (21 filters)	6 colors (21 filters)	5 colors	6 colors	6 colors
Temperature Zones	3 VeriFlex zones	6 VeriFlex zones	3 VeriFlex zones	6 VeriFlex zones	Gradient	Gradient	Gradient
Multiplexing Capability	Up to 4 targets	Up to 6 targets	Up to 5 targets	Up to 6 targets	Up to 5 targets	Up to 6 targets	Up to 6 targets
Light Source	White LED	White LED	White LED	White LED	Halogen	LED	LED
Software	Simplified Design & Analysis	Advanced Design & Analysis	Advanced, smart-enabled	Advanced, smart-enabled	Bio-Rad CFX Maestro	Roche LC software	Agilent AriaMx software
Connectivity	USB, Wi-Fi, Ethernet	USB, Wi-Fi, Ethernet	USB, Wi-Fi, Ethernet	USB, Wi-Fi, Ethernet	USB, Ethernet	USB	USB, Wi-Fi, Ethernet
Calibration	Factory-calibrated	Factory-calibrated	Factory-calibrated	Factory-calibrated	User-calibrated	Factory-calibrated	User-calibrated
Dynamic Range	10 logs	10 logs	10 logs	10 logs	9 logs	10 logs	10 logs
Sensitivity	1.5-fold changes	1.5-fold changes	1.5-fold changes	1.5-fold changes	1.5-fold changes	1.5-fold changes	1.5-fold changes
Security Features	None	21 CFR Part 11 compliance	21 CFR Part 11 compliance	21 CFR Part 11 compliance	None	None	None
Special Features	Compact, affordable	Multiplexing, compliance	Interchangeable blocks, smart features	Interchangeable blocks, smart features	Gradient, affordable	High-throughput	Modular design
Applications	Basic research, education	Clinical research, diagnostics	Flexible lab workflows, scalable	High-throughput diagnostics	Research and diagnostics	High-throughput research	Research and diagnostics

Summary of Comparisons

QuantStudio 3: Ideal for basic research and teaching labs with limited budgets.
QuantStudio 5: Offers advanced multiplexing, CFR compliance, and expanded applications for research and diagnostics.
QuantStudio 6 Pro: Flexible with interchangeable blocks and smart-enabled features for adaptable workflows.
QuantStudio 7 Pro: Advanced capabilities with high multiplexing and features for regulated environments.
Bio-Rad CFX96 Touch: A robust and affordable gradient-enabled system for research labs.
Roche LightCycler 480 II: High-throughput capabilities and robust performance for complex applications.
Agilent AriaMx: Modular design with strong multiplexing and gradient capabilities for diverse genomic applications.

This table provides a comprehensive comparison to assist in selecting the most suitable real-time PCR system for your specific laboratory needs.