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Applied Biosystems™ MicroAmp™ Optical Adhesive Films
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Plate Compatibility: 384-Well Plates, 96-Well Plates
Specifications:
| Application | qPCR |
| Material | Polyester |
| Storage Temperature | Ambient |
| Product Type | Microplate sealer |
| Product Brand | Thermo Fisher Scientific™ |
| Product Grade | Molecular Biology |
The Applied Biosystems MicroAmp Optical Adhesive Film is a high-quality sealing solution for PCR and qPCR workflows. It is specifically designed to minimize well-to-well contamination and prevent sample evaporation while maintaining optical clarity for real-time PCR applications. Its pressure-sensitive adhesive ensures a tight, reliable seal, making it a valuable accessory for molecular biology workflows.
Key Features
- Pressure-Sensitive Adhesive:
- Provides a tight seal over every well, minimizing cross-contamination and sample loss.
- Ensures consistent and accurate results in real-time PCR.
- Optical Clarity:
- Does not interfere with fluorescence-based sample readings, making it ideal for qPCR.
- User-Friendly Design:
- Easy-to-apply by peeling away the backing and pressing firmly across the plate.
- Non-sticky design prevents adhesion to gloves during application.
- Stackable Plates:
- The protective covering allows for easy stacking of microplates, saving space in storage.
- Plate Compatibility:
- Designed to fit 96-well and 384-well MicroAmp plates.
- Suitable for use with a wide range of Applied Biosystems thermal cyclers.
Specifications
| Feature | Details |
|---|---|
| Color | Optical |
| Material | Polyester |
| Adhesive | Pressure-sensitive |
| Plate Compatibility | 96-well and 384-well MicroAmp plates |
| No. per Case | 100 covers |
| For Use With Equipment | Applied Biosystems thermal cyclers, including QuantStudio™ and StepOnePlus™ |
| Shipping Condition | Room temperature |
| Storage | Room temperature |
| Product Type | Film, Seal, or Applicator |
| Unit Size | 100 covers |
Applications
- Real-Time PCR (qPCR):
- Ideal for fluorescence-based applications where optical clarity is critical.
- PCR Thermal Cycling:
- Prevents evaporation and cross-contamination during thermal cycling.
- Sample Storage:
- Maintains sample integrity during short-term storage.
- High-Throughput Workflows:
- Compatible with both 96- and 384-well plates for scalable PCR applications.
Compatibility
Thermal Cyclers:
- Compatible with a wide range of Applied Biosystems systems, including:
- QuantStudio™ Dx, QuantStudio™ systems.
- 7500 Fast Dx, 7500 Fast System, StepOnePlus™, Veriti™, ViiA™ 7 Systems.
- ProFlex™, GeneAmp™, and SimpliAmp™ Thermal Cyclers.
Plate Types:
- Designed for MicroAmp 96-well and 384-well plates.
Advantages
- Evaporation Control: Ensures high sample recovery by preventing evaporation during thermal cycling.
- Optical Clarity: Suitable for qPCR applications without interfering with fluorescence detection.
- Ease of Use: Quick application and removal with minimal effort.
- Stackability: Protective design allows efficient stacking for storage.
Why Choose MicroAmp Optical Adhesive Film?
- Specifically optimized for real-time PCR and qPCR workflows.
- Provides reliable sealing and consistent results.
- Compatible with a wide range of Applied Biosystems thermal cyclers.
When selecting adhesive films for PCR applications, two notable options are the Thermo Scientific™ Adhesive PCR Plate Seals and the Applied Biosystems™ MicroAmp™ Optical Adhesive Films. Below is a detailed comparison of their features:
| Feature | Thermo Scientific™ Adhesive PCR Plate Seals | Applied Biosystems™ MicroAmp™ Optical Adhesive Films |
|---|---|---|
| Material | Clear adhesive film for sample inspection | Optically clear polyester film for real-time PCR applications |
| Adhesion | Strong adhesive prevents evaporation during thermal cycling | Pressure-sensitive adhesive ensures a tight seal and reduces contamination |
| Residue | Leaves no sticky residue when removed | Prevents sticking to gloves for clean handling |
| Application | End tabs simplify application and removal | Peel-and-press application over the plate |
| Temperature Range | -20°C to 120°C | Suitable for standard PCR cycling (specific range not specified) |
| Compatibility | Works with screw-down or clip-down lid cyclers | Tailored for MicroAmp 96- and 384-well plates, may fit others |
| Plate Compatibility | Compatible with 96- and 384-well plates | Designed for MicroAmp plates (96- and 384-well) |
| Optical Clarity | Clear film for general sample inspection | Optically clear for fluorescence-based real-time PCR |
| Packaging | 100 sheets per pack | 100 covers per pack |
| Intended Use | General PCR and sample storage | Real-time PCR and fluorescence-based applications |
Summary
- Choose Thermo Scientific™ Adhesive PCR Plate Seals if:
- You need versatility for both general PCR and sample storage.
- You require a broader temperature range (-20°C to 120°C).
- End-tab design simplifies handling.
- Choose Applied Biosystems™ MicroAmp™ Optical Adhesive Films if:
- You are performing real-time PCR or fluorescence-based applications.
- Optical clarity is critical for fluorescence readings.
- You are using MicroAmp plates and require a tailored fit.
This adhesive film is a premium sealing solution for PCR and qPCR workflows, offering precision, ease of use, and compatibility with standard lab equipment.