Applied Biosystems™ QuantStudio™ 3 Real-Time PCR System, 96-well, 0.2 mL, laptop

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Specifications:

Application	qPCR	Warranty	1 Year
Storage Temperature	Room Temperature
Product Type	thermal Cycler
Product Brand	Thermo Fisher Scientific™
Product Grade	Molecular Biology

Overview
Resource

The QuantStudio™ 3 Real-Time PCR System offers affordability, ease of use, and reliable performance for researchers of all experience levels. It combines proven OptiFlex technology, cloud connectivity, and intuitive software to deliver high-quality qPCR data for a wide range of genomic applications.

Key Features and Benefits

Proven OptiFlex Technology:
- Four coupled excitation and emission filter channels with white LED.
- Delivers improved well-to-well and instrument-to-instrument data accuracy.
- Offers enhanced sensitivity to detect small target quantity changes (as small as 1.5-fold).
VeriFlex Temperature Control:
- Features three independent temperature zones for precise optimization of PCR conditions.
Interactive Touchscreen:
- Simplified instrument software for easy navigation.
- Pre-optimized protocol templates for quick experiment setup.
Cloud-Enabled Connectivity with iConnect:
- Upload, store, and analyze data securely from anywhere with internet access.
- Flexible web browser-based qPCR analysis modules for quick and efficient data handling.
Software Options:
- Design and Analysis Cloud Software:
  - Accessible via web browser (no software installation required).
  - Provides real-time data access, no licenses needed.
- Design and Analysis Desktop Software:
  - Traditional PC-based system configuration for localized use.
  - No additional license costs.
Compact and User-Friendly:
- Factory-calibrated and delivered in easy-to-use packaging for quick installation.
- Compact design allows placement in limited bench spaces.
High Application Versatility:
- Supports diverse genomic applications including:
  - Gene expression
  - Genetic variation
  - Gene regulation
  - SNP genotyping
  - MicroRNA profiling

Technical Specifications

Feature	Details
Block Configuration	96-well, 0.2 mL format (also available in 0.1 mL)
Temperature Zones	3 independent VeriFlex zones
Dynamic Range	10 logs
Detection Sensitivity	Detect 1.5-fold changes in target quantity
Filter Channels	4 coupled excitation/emission filter channels
Light Source	White LED
Software Options	Cloud-based or desktop Design and Analysis software
Connectivity	iConnect, Wi-Fi, USB, Ethernet
Operating System	Windows 10 (desktop software)
Display	Interactive touchscreen
Dimensions	Compact design
Weight	Lightweight and portable
Calibration	Factory calibrated

Applications

Gene Expression Analysis: Quantify mRNA and study gene regulation.
Genetic Variation Studies: SNP genotyping and allele discrimination.
MicroRNA Profiling: Detect and analyze microRNA expression levels.
High-Resolution Melt Analysis (HRM): Study epigenetic changes and mutation detection.
Pathogen Detection: Identify and quantify pathogen DNA/RNA in samples.

Included Components

QuantStudio 3 Real-Time PCR System
Laptop (pre-configured for system control)
Power cords and accessories

Why Choose the QuantStudio™ 3 System?

Affordable and Compact: Ideal for labs with budget and space constraints.
Flexible and Accessible: Cloud-enabled for remote data access and management.
User-Friendly: Simplified workflows and pre-optimized protocols for seamless operation.
Proven Technology: Trusted OptiFlex and VeriFlex technologies ensure accuracy and reliability.
Versatile Applications: Suitable for a wide range of genomic studies.

When selecting a real-time PCR system, it's essential to compare various models to identify the one that best fits your laboratory's needs. Below is a detailed comparison of the Applied Biosystems™ QuantStudio™ 3 Real-Time PCR System with its counterparts, the QuantStudio™ 1 and QuantStudio™ 5, as well as comparable models from other leading manufacturers.

Comparison Table:

Feature/Model	QuantStudio™ 1	QuantStudio™ 3	QuantStudio™ 5	Bio-Rad CFX96 Touch	Roche LightCycler 96	Agilent AriaMx
Block Configuration	96-well	96-well	96-well or 384-well	96-well	96-well	96-well
Temperature Zones	Single	3 VeriFlex zones	6 VeriFlex zones (96-well)	Gradient	Gradient	Gradient
Optical Channels	4	4	6 (96-well) or 5 (384-well)	5	6	6
Light Source	LED	White LED	White LED	Halogen	LED	LED
Dynamic Range	9 logs	10 logs	10 logs	9 logs	10 logs	10 logs
Sensitivity	Detects 1.5-fold changes	Detects 1.5-fold changes	Detects 1.5-fold changes	Detects 1.5-fold changes	Detects 1.5-fold changes	Detects 1.5-fold changes
Software Interface	Touchscreen	Touchscreen	Touchscreen	Touchscreen	PC-based	Touchscreen
Connectivity	USB	USB, Wi-Fi, Ethernet	USB, Wi-Fi, Ethernet	USB, Wi-Fi	USB, Ethernet	USB, Wi-Fi, Ethernet
Dimensions (W × D × H)	27 × 50 × 40 cm	27 × 50 × 40 cm	27 × 50 × 40 cm	33 × 46 × 48 cm	37 × 47 × 40 cm	33 × 46 × 48 cm
Weight	25 kg	25 kg	25 kg	32 kg	35 kg	30 kg
Multiplexing Capability	Up to 4 targets	Up to 4 targets	Up to 6 targets (96-well)	Up to 5 targets	Up to 6 targets	Up to 6 targets
Calibration	Factory-calibrated	Factory-calibrated	Factory-calibrated	User-calibrated	Factory-calibrated	User-calibrated
Applications	Gene expression, genotyping	Gene expression, genotyping, pathogen detection	Gene expression, genotyping, pathogen detection, high-throughput screening	Gene expression, genotyping, pathogen detection	Gene expression, genotyping, pathogen detection	Gene expression, genotyping, pathogen detection
Additional Features	-	Cloud-enabled	Cloud-enabled, 21 CFR Part 11 compliance	Gradient optimization	High-resolution melting	Modular design

Key Considerations:

Temperature Control: The QuantStudio™ 3 and 5 models feature VeriFlex technology, providing multiple independent temperature zones for precise PCR optimization. In contrast, the QuantStudio™ 1 offers a single temperature zone. Models like the Bio-Rad CFX96 Touch and Roche LightCycler 96 offer gradient capabilities for temperature optimization.
Optical Channels and Multiplexing: The QuantStudio™ 5 offers up to 6 optical channels (in the 96-well configuration), enabling higher multiplexing capabilities compared to the QuantStudio™ 3 and 1, which offer up to 4 channels. This allows for the detection of more targets per run.
Connectivity and Software: The QuantStudio™ 3 and 5 systems are cloud-enabled, allowing for remote data access and analysis via Thermo Fisher's cloud platform. This feature is particularly beneficial for collaborative environments and data management.
Applications and Flexibility: The QuantStudio™ 5 system supports both 96-well and 384-well formats, offering greater flexibility for varying throughput needs.
Calibration and Maintenance: Factory calibration in the QuantStudio™ systems ensures consistent performance and reduces the need for user intervention. Some competitor models require user calibration, which may necessitate additional maintenance.

When choosing a real-time PCR system, consider factors such as throughput requirements, multiplexing needs, connectivity preferences, and budget constraints. The QuantStudio™ series offers a range of options to accommodate different laboratory needs, with the QuantStudio™ 3 providing a balance of performance and affordability, and the QuantStudio™ 5 offering enhanced features for more demanding applications.