Invitrogen™ Attune™ NxT Flow Cytometer, blue/red/violet (3 Lasers)

High-Speed, Modular Flow Cytometry with Acoustic Focusing Technology

The Invitrogen™ Attune™ NxT Flow Cytometer is a high-performance, 3-laser flow cytometry platform that integrates acoustic focusing technology with advanced optics and flexible software to enable rapid, reliable, and high-parameter analysis of diverse biological samples. With blue (488 nm), red (638 nm), and violet (405 nm) excitation lasers, it supports up to 14 fluorescent colors and 16 total parameters, offering powerful multicolor capabilities for both routine and complex flow cytometry applications.

The instrument is built with a modular and upgradable design, allowing researchers to add additional lasers and filters as their experimental needs grow. Whether you're working with limited, precious samples or performing high-throughput analysis, the Attune™ NxT delivers speed, flexibility, and consistency.

Key Features and Benefits

• Acoustic Focusing Technology
  Aligns cells using ultrasonic waves instead of purely hydrodynamic forces, reducing sample pressure and improving speed, alignment, and data quality.
• Fast Sample Acquisition
  Processes samples at speeds up to 35,000 events/sec, with maximum electronics support of 65,000 events/sec, allowing efficient data collection even from dilute or small-volume samples.
• Three-Laser Configuration
  Equipped with blue (488 nm), red (638 nm), and violet (405 nm) lasers, the system supports 14-color panels and a broad range of fluorophores.
• Modular and Upgradeable
  Start with 1–3 lasers and expand up to 4 as needed. Add lasers and customize optical filters without replacing the instrument.
• Compact Footprint
  Small enough to fit on a benchtop or within a biosafety cabinet, ideal for labs with limited space.
• 21 CFR Part 11 Compliance
  Optional software package for regulated environments, compatible with Windows 10 (64-bit), supports audit trails, user roles, and data security.
• Enhanced Workflow Tools
  Compatible with CytKick™ and CytKick™ Max autosamplers for high-throughput workflows. Easily define acquisition protocols and settings for tubes or plates.
• Sample Versatility
  Suitable for analysis of whole blood, CSF, stem cells, bone marrow, mouse blood, and rare populations, using syringe-driven sample delivery (up to 75 psi) for clog-resistant operation.
• No-Wash, No-Lyse Compatibility
  Compatible with the Attune™ NxT No-Wash No-Lyse Filter Kit, enabling gentle analysis of whole blood without red blood cell lysis or centrifugation.

Common Applications

• Immunophenotyping: Identification and characterization of immune cell subsets
• Stem Cell Analysis: Detection of rare progenitor populations
• Cell Cycle and Apoptosis: Quantification of DNA content and apoptotic markers
• Rare Event Detection: Monitoring low-frequency events like CTCs or MRD
• Signaling and Cytokine Assays: Phospho-flow, intracellular staining
• Microbial Analysis: Enumeration and viability of yeast, bacteria, and fungi
• Small-Volume or Dilute Sample Analysis: Useful for pediatric samples or tissue aspirates

Technical Specifications

Kit Contents

• Attune™ NxT Flow Cytometer (3-laser configuration)
• Minitower computer with Windows 10 64-bit and Attune™ Cytometric Software
• Power cord with universal plug adapters
• USB cable
• Attune™ Shutdown Solution
• Attune™ Wash Solution
• Attune™ Focusing Fluid
• Flow Cell Cleaning Solution
• Attune™ Performance Tracking Beads
• Quick Reference Card

The Invitrogen™ Attune™ NxT Flow Cytometer (Blue/Red/Violet configuration) is a powerful and flexible flow cytometry solution engineered to meet the evolving needs of modern research laboratories. Its high-speed acoustic focusing, three-laser detection system, and modular expandability make it ideal for both routine and advanced applications across immunology, oncology, stem cell biology, and microbiology.

With capabilities designed for speed, precision, and adaptability, this cytometer ensures high-quality data from diverse samples while optimizing user efficiency and sample preservation. Whether you're conducting multicolor immunophenotyping or analyzing rare populations in complex matrices, the Attune™ NxT sets a new standard for performance and usability in flow cytometry.