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Invitrogen™ ATP Determination Kit, 200–1000 assays
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Specifications:
| Application | Fluorescence-Based Assay | ||
| Storage Temperature | -5 to -30°C | ||
| Product Type | Assay Kits | Forms | Kit with Various components |
| Product Brand | Thermo Fisher Scientific™, Invitrogen | ||
| Product Grade | Molecular Biology | ||
The Invitrogen™ ATP Determination Kit is a highly sensitive luminescence-based assay for the quantitative detection of ATP in biochemical assays, cell viability tests, metabolic studies, and microbial detection. Leveraging the luciferase–luciferin reaction, this kit can detect as little as 0.1 picomole of ATP using a luminometer or microplate reader equipped with luminescence capability.
With its exceptional sensitivity and flexible format, the kit is ideal for applications ranging from cell viability analysis to environmental contamination monitoring. The luciferase and luciferin components are supplied separately, enabling optimization for specific sample types and detection instruments.
Assay Principle
This assay is based on the absolute requirement of luciferase for ATP to generate light. In the presence of Mg²⁺, luciferase catalyzes the reaction of D-luciferin, ATP, and O₂ to form oxyluciferin, AMP, CO₂, pyrophosphate, and light emission at ~560 nm (pH 7.8). The luminescence signal is directly proportional to ATP concentration, allowing precise quantification.
Key Features
- Exceptional Sensitivity – Detects as little as 0.1 picomole of ATP.
- Flexible Throughput – Perform 200–1,000 assays depending on format (tube or microplate).
- Customizable Assay Conditions – Separate luciferase and luciferin for optimal performance.
- Wide Sample Compatibility – Works with extracellular ATP or cell lysates.
- Generous Kit Supply – Enough reagents for ten 96-well plates.
- Broad Research Applications – Suitable for biochemical, environmental, and microbiological studies.
Applications
- Cell Viability & Cytotoxicity Testing – Assess metabolic activity in mammalian or microbial cells.
- Microbial Detection – Monitor bacterial and fungal contamination in clinical and environmental samples.
- Metabolism & Bioenergetics Research – Evaluate mitochondrial activity and ATP production.
- Enzyme Activity Assays – Study ATPases, NADPH oxidases, and other ATP-dependent enzymes.
- Antibiotic & Drug Screening – Measure effects on ATP levels to differentiate cytostatic from cytocidal effects.
- Environmental Testing – Detect ATP in water, soil, food, and industrial samples.
Technical Specifications
| Parameter | Specification |
|---|---|
| Detection Method | Bioluminescence, Chemiluminescence |
| Format | Tube or 96-well plate |
| Label Type | Luciferin |
| Substrate | D-Luciferin |
| Sensitivity | 0.1 picomole ATP |
| Emission | ~560 nm (pH 7.8) |
| Kit Capacity | 200–1,000 assays |
| For Use With | Luminometer (microplate or tube-based), Microplate Reader |
| Storage | –5°C to –30°C, protect from light |
| Shipping Condition | Wet Ice |
| Unit Size | Each |
Kit Contents
- Recombinant firefly luciferase
- D-Luciferin substrate
- 10X Reaction Buffer
- Substrate Solvent
- Fluorescent ATP Standard
- Detailed protocol
The Invitrogen™ ATP Determination Kit delivers exceptional sensitivity, flexibility, and reproducibility for ATP quantification in a wide variety of research applications. From cell viability assessment to environmental testing, this kit is a gold-standard luminescent assay for precise ATP measurement.