Invitrogen™ H2DCFDA (H2-DCF, DCF), 100mg

The 2',7'-dichlorodihydrofluorescein diacetate (H2DCFDA), also known as dichlorofluorescin diacetate, is a cell-permeant indicator widely used to detect reactive oxygen species (ROS) in live cells. Its versatile applications include monitoring oxidative stress, detecting ROS intermediates, and studying cellular responses to environmental or physiological changes, such as ferroptosis.

Key Features

1. ROS Detection:
   • Sensitive detection of intracellular reactive oxygen species, including singlet oxygen, superoxide, hydroxyl radicals, and peroxides.
2. Cell-Permeant Indicator:
   • Easily enters cells, where intracellular esterases cleave acetate groups, and ROS oxidize the molecule to the fluorescent 2',7'-dichlorofluorescein (DCF).
3. High Fluorescence Sensitivity:
   • Detectable fluorescence with excitation/emission wavelengths of 492–495 nm/517–527 nm.
4. Versatile Applications:
   • Suitable for flow cytometry, fluorometry, fluorescence microscopy, and microplate reader analysis.
5. Flexible Usage:
   • Can be dissolved in DMSO, DMF, or ethanol for experimental applications.
6. Enhanced Retention Versions:
   • Modified variants (C400, C2938) with better cellular retention are available.

Specifications

Applications

1. Reactive Oxygen Species Detection:
   • Study oxidative stress in live cells.
   • Monitor reactive oxygen intermediates in immune cells like neutrophils and macrophages.
2. Cell Death Mechanisms:
   • Investigate ferroptosis and iron-dependent cell death mechanisms.
3. Environmental and Physiological Studies:
   • Examine cellular responses to oxidative environments.
4. Fluorescent Analysis:
   • Analyze ROS activity using advanced imaging and quantification techniques.
5. Comparative ROS Studies:
   • Utilize enhanced retention versions (C400, C2938) for longer-term cellular retention studies.

Advantages

Recommended Protocols

1. Dissolve Indicator:
   • Prepare H2DCFDA in DMSO, DMF, or ethanol at desired concentrations.
2. Cell Loading:
   • Incubate cells with H2DCFDA under optimized conditions.
   • Follow literature-specific protocols for cell type and experimental needs.
3. ROS Detection:
   • Use compatible equipment:
     • Flow cytometer for population-level analysis.
     • Fluorometer or microplate reader for quantitative measurements.
     • Fluorescence microscope for localized ROS visualization.

Additional Products for ROS Research

• ROS Generation Reagents:
  • For controlled ROS induction.
• Other ROS Indicators:
  • Singlet oxygen, superoxide, hydroxyl radical, and peroxide-specific probes.
• Ferroptosis Research Tools:
  • Comprehensive kits for studying iron-dependent cell death.

Competitor Comparison

Comparison of H2DCFDA ROS Indicators Across Brands