Q-Plex™ Human Environmental Enteric Dysfunction (11-Plex)

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For measurement of AGP, CD-14, CRP, Ferritin, FGF-21, HRP2, IFABP, IGF-1, RBP4, sTfR, Thyroglobulin

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Specifications:

Application	Protein Assays & Analysis
Storage Temperature	2-8°C
Product Type	Assay Kits	Forms	Kit with Various components
Product Brand	QUANSYS Biosciences
Product Grade	Molecular Biology

Overview
Resource

The Q-Plex Human Environmental Enteric Dysfunction (11-Plex) is a fully quantitative, planar multiplex chemiluminescent immunoassay designed for simultaneous measurement of eleven biomarkers associated with Environmental Enteric Dysfunction (EED), nutrition, systemic inflammation, intestinal integrity, growth, and malaria status.

Developed in collaboration with PATH, this multiplex array enables researchers to generate a comprehensive nutritional and inflammatory biomarker profile using only 5 µL of serum or heparin plasma per well. The assay is optimized for high-throughput testing, delivering quantitative results within 3.5 hours with <1% cross-reactivity and no significant observed interference.

Background – Environmental Enteric Dysfunction (EED)

Environmental Enteric Dysfunction is characterized by chronic intestinal inflammation, compromised mucosal barrier function, and impaired nutrient absorption. It is highly prevalent in low-resource settings with high enteric pathogen exposure and inadequate sanitation. EED is associated with:

Stunted growth
Impaired cognitive development
Reduced vaccine responsiveness
Increased susceptibility to infections such as pneumonia, diarrhea, and malaria

The Q-Plex EED (11-Plex) panel provides an integrated biomarker solution to support population health research, clinical studies, and global health programs focused on malnutrition and childhood disease risk.

Target Analytes and Clinical Relevance

Growth and Vaccine Response

Intestinal Fatty Acid Binding Protein (I-FABP) – Marker of small intestine damage
Soluble CD14 (sCD14) – White blood cell activation
Insulin-like Growth Factor 1 (IGF-1) – Bone and tissue growth
Fibroblast Growth Factor 21 (FGF21) – Metabolic pathway modulation

Systemic Inflammation

Alpha-1-acid Glycoprotein (AGP) – Inflammatory response
C-reactive Protein (CRP) – Acute-phase inflammation

Nutritional Status

Ferritin – Iron storage
Soluble Transferrin Receptor (sTfR) – Iron deficiency
Retinol Binding Protein 4 (RBP4) – Vitamin A transport
Thyroglobulin (Tg) – Iodine nutritional status

Malaria Indicator

Histidine-Rich Protein 2 (HRP2) – Presence of malaria infection

Assay Principle

The Q-Plex EED panel utilizes a planar multiplex array format in which capture antibodies are spatially immobilized at defined positions within each well. Both competitive and sandwich immunoassay formats are integrated within the same plate depending on the analyte.

Following sample incubation, detection reagents and Streptavidin-HRP enable chemiluminescent signal generation. Light emission is captured using Q-View imagers and analyzed with Q-View Software for fully quantitative reporting.

Cross-reactivity is <1% across analytes, ensuring reliable multiplex performance.

Performance and Validation Data

Analyte	Assay Type	Calibrator Range	ULOQ	LLOQ	LOD	Inter-Assay CV	Intra-Assay CV	Avg. Linearity
AGP	Competitive	0.36 – 0.00049 g/L	0.25 g/L	0.0019 g/L	0.00025 g/L	12%	5%	107%
CD-14	Competitive	1,750 – 2.4 ng/mL	1,225 ng/mL	9.4 ng/mL	1.2 ng/mL	11%	6%	93%
CRP	Competitive	5.61 – 0.0077 mg/L	3.93 mg/L	0.03 mg/L	0.0038 mg/L	14%	5%	95%
Ferritin	Sandwich	115 – 0.16 µg/L	80.5 µg/L	0.21 µg/L	0.079 µg/L	12%	8%	102%
FGF-21	Sandwich	800 – 1.10 pg/mL	560 pg/mL	1.40 pg/mL	0.55 pg/mL	12%	6%	100%
HRP2	Sandwich	0.96 – 0.0013 µg/L	0.67 µg/L	0.0017 µg/L	0.00066 µg/L	13%	4%	100%
IFABP	Sandwich	3,000 – 4.12 pg/mL	2,100 pg/mL	5.3 pg/mL	2.06 pg/mL	10%	5%	92%
IGF-1	Sandwich	198 – 0.27 ng/mL	139 ng/mL	0.35 ng/mL	0.14 ng/mL	13%	9%	118%
RBP4	Competitive	1.4 – 0.0019 µmol/L	0.98 µmol/L	0.0075 µmol/L	0.0010 µmol/L	11%	8%	93%
sTfR	Sandwich	108 – 0.15 mg/L	75.3 mg/L	0.19 mg/L	0.074 mg/L	15%	6%	115%
Thyroglobulin	Sandwich	13.7 – 0.019 µg/L	9.59 µg/L	0.024 µg/L	0.0094 µg/L	13%	4%	100%

Cross-Reactivity and Interference: <1%; no significant interference observed.

Validated Sample Types

Human Serum
Human Heparin Plasma

Kit Contents

Each kit includes:

96-well multiplex plate pre-coated with 11 biomarkers
Calibrator standards
Competitor reagents (for competitive assays)
Detection mix
Streptavidin-HRP
Chemiluminescent substrates
Sample diluents
Wash buffer
Instruction manual

Required Instrumentation

All Q-Plex multiplex assays require compatible Q-View imagers for signal detection and Q-View software for data analysis. Performance with non-compatible imaging systems is not guaranteed.

Applications

Environmental Enteric Dysfunction research
Child growth and nutrition studies
Vaccine response investigations
Global health and public health surveillance
Malaria epidemiology studies
Micronutrient deficiency research
Population-level biomarker screening

The Q-Plex Human EED (11-Plex) panel provides an integrated, small-volume, high-sensitivity multiplex solution for simultaneous assessment of inflammation, nutrition, intestinal damage, growth markers, and malaria exposure. The planar multiplex design eliminates bead-based complexity while maintaining robust precision and minimal cross-reactivity.