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Invitrogen™ ProcartaPlex™ Mouse Essential Th1/Th2 Cytokine Panel 2, 8plex, 96 Tests
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Target list [bead region]: GRO alpha (CXCL1) [43], IL-1 beta [19], IL-10 [13], IL-17A (CTLA-8) [52], MCP-1 (CCL2) [51], MIP-1 alpha (CCL3) [47] ,MIP-1 beta (CCL4) [72], MIP-2 [55]
Specifications:
| Application | Protein Assays & Analysis, Luminex Assays | ||
| Storage Temperature | 2-8°C | ||
| Product Type | Luminex Assays | Forms | Kit with Various components |
| Product Brand | Thermo Fisher Scientific™, Invitrogen | ||
| Product Grade | Molecular Biology | ||
Expanded multiplex assay for Th1/Th2 cytokine profiling in mouse models
The Invitrogen™ ProcartaPlex™ Mouse Essential Th1/Th2 Cytokine Panel 2, 8plex is designed to detect and quantify eight critical cytokines in a single well using Luminex™ xMAP technology. This high-throughput multiplex panel supports immune response profiling, biomarker discovery, and validation studies, enabling researchers to analyze multiple immune mediators from minimal sample volumes.
By simultaneously measuring GRO alpha (CXCL1), IL-1 beta, IL-10, IL-17A, MCP-1, MIP-1 alpha, MIP-1 beta, and MIP-2, the panel provides an extended view of Th1/Th2-associated cytokine expression, complementing the Mouse Essential Th1/Th2 Cytokine Panel, 6plex for broader coverage.
Key Features & Benefits
- Expanded Multiplex Detection – Measure 8 Th1/Th2 cytokines in one well.
- Sample-Efficient – Requires only 25 μL of serum or plasma, or 50 μL of cell culture supernatant.
- Validated for Performance – Extensively tested for combinability, minimal cross-reactivity, and analyte stability.
- Rapid Turnaround – Obtain results in ~4 hours using standard Luminex platforms.
- Complementary Panel Design – Works alongside the Mouse Essential Th1/Th2 Cytokine Panel, 6plex for a total of 14 cytokines.
- Flexible Research Applications – Suitable for immunology, inflammation, and vaccine studies.
Applications
- Immunology Research – Comprehensive Th1/Th2 cytokine profiling.
- Inflammatory Disease Studies – Analysis of pro- and anti-inflammatory cytokine patterns.
- Vaccine Research – Evaluation of immune response profiles post-immunization.
- Preclinical Mouse Models – Immune modulation studies in infectious or autoimmune disease models.
Target Analytes & Bead Regions
| Cytokine | Bead Region |
|---|---|
| GRO alpha (CXCL1) | 43 |
| IL-1 beta | 19 |
| IL-10 | 13 |
| IL-17A (CTLA-8) | 52 |
| MCP-1 (CCL2) | 51 |
| MIP-1 alpha (CCL3) | 47 |
| MIP-1 beta (CCL4) | 72 |
| MIP-2 | 55 |
Technical Specifications
| Property | Details |
|---|---|
| Format | Multiplex Kit |
| Technology | Luminex™ xMAP |
| Sample Types | Serum, Plasma, Cell Culture Supernatants |
| Sample Volume | Serum/Plasma: 25 μL; CCS: 50 μL |
| Assay Time | ~4 hours |
| Assay Sensitivity & Range | See Certificate of Analysis |
| For Use With | Luminex 200, FLEXMAP 3D, MAGPIX |
| Species | Mouse |
| Combinability | Combinable |
| Research Areas | Immunology, Cytokines |
| Shipping | Wet Ice |
| Storage | 2–8 °C |
Kit Contents
- 2 vials Mouse Standard Mix A (lyophilized)
- 1 vial Capture Bead Mix (1X)
- 1 vial Biotinylated Detection Antibody Mix (50X)
- 1 bottle Reading Buffer (1X)
- 1 bottle Wash Buffer (10X)
- 1 bottle Streptavidin-PE (1X)
- 1 bottle Universal Assay Buffer (1X)
- 1 bottle Detection Antibody Diluent (1X)
- 8-tube strip
- Adhesive film
- Flat-bottom 96-well black plate
Ordering Information
| Catalog Number | Quantity |
|---|---|
| EPX080-20832-901 | 96 Tests |
The Invitrogen™ ProcartaPlex™ Mouse Essential Th1/Th2 Cytokine Panel 2, 8plex offers a powerful, validated, and efficient solution for multi-analyte cytokine detection in mouse samples. Its expanded target list, low sample requirements, and compatibility with standard Luminex platforms make it an essential tool for advanced immune profiling, biomarker validation, and preclinical research. When combined with the 6plex panel, researchers can achieve a comprehensive 14-cytokine Th1/Th2 profile, maximizing experimental insight while conserving resources.