Thermo Scientific™ Pierce™ Protein A/G IgG Binding Buffer, 240mL, pH 8.0; contains EDTA as a preservative

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Specifications:

Application	Protein Assays & Analysis
Storage Temperature	2-8°C
Product Type	Buffer	Forms	Liquid
Product Brand	Thermo Fisher Scientific™
Product Grade	Molecular Biology

Overview
Resource

Thermo Scientific™ Pierce™ Protein A/G IgG Binding Buffer is a phosphate-based buffer formulated at pH 8.0, optimized for IgG antibody binding to Protein A, Protein G, or Protein A/G agarose resins. This versatile solution contains EDTA for enhanced protein stability and is designed to support non-denaturing antibody purification workflows.

The buffer ensures efficient and selective IgG binding across multiple subclasses and species, making it a preferred solution when using Protein A/G mixed resins in purifications involving complex or unknown antibody isotypes. It is supplied prefiltered, preformulated, and ready for immediate use.

Key Features & Benefits

Optimized for Protein A/G Binding
Ideal for workflows involving Protein A, Protein G, or Protein A/G supports.
Phosphate-Based, pH 8.0
Maintains native antibody conformation and binding efficiency under physiological pH.
Stabilizing Additive
Includes EDTA to inhibit metal-dependent proteases and maintain protein integrity.
Ready-to-Use Format
Preformulated and prefiltered 1X solution eliminates buffer preparation steps.
Cross-Platform Compatibility
Compatible with Protein A, Protein G, and combination resins used in manual and automated antibody purification systems.

Technical Specifications

Property	Specification
Product Name	Protein A/G IgG Binding Buffer
Catalog Number	54200
pH	8.0
Buffer Composition	Phosphate-based, contains EDTA
Form	Liquid, 1X ready-to-use
Volume	240 mL
Reagent Type	Binding Buffer
Compatibility	Protein A, Protein G, and Protein A/G supports
Storage Conditions	Store at 4°C
Intended Use	For Research Use Only (RUO)

Applications

IgG Binding & Purification
Suitable for purifying monoclonal and polyclonal IgG using Protein A, G, or A/G supports.
Antibody-Antigen Complex Capture
Effective in workflows requiring Fc-region-specific affinity binding.
Flexible Immunoprecipitation Protocols
Supports workflows involving mixed-species IgG subclasses with varying Protein A/G affinities.
Protein Interaction Studies
Provides consistent binding conditions for immunoprecipitation and co-IP assays.

Thermo Scientific™ Pierce™ Protein A/G IgG Binding Buffer offers a robust, ready-to-use solution for high-efficiency antibody binding in Protein A/G-based affinity purification workflows. Its pH-optimized, phosphate-buffered, and EDTA-stabilized formulation ensures reproducible results in IgG capture, protein interaction studies, and antibody isolation across a wide range of sample types and experimental conditions.