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Thermo Scientific™ Pierce™ Protein A/G Plus Agarose
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Specifications:
| Application | Immunohistochemistry, Protein Purification | ||
| Storage Temperature | 2-8°C | ||
| Product Type | Molecular Biology Reagent | Forms | Liquid |
| Product Brand | Thermo Fisher Scientific™ | ||
| Product Grade | Molecular Biology | ||
Thermo Scientific™ Pierce™ Protein A/G Plus Agarose is an exceptionally high-capacity affinity resin designed for efficient and selective purification of IgG antibodies across a wide range of mammalian species. This Plus-grade resin features a dense immobilization of recombinant Protein A/G on a robust, crosslinked 6% agarose matrix, delivering unmatched performance for demanding antibody purification applications.
Key Features & Benefits
-
Ultra-High Binding Capacity
Binds >50 mg of human IgG per mL of resin, making it ideal for high-yield antibody purification. -
Fusion Protein for Broad IgG Recognition
Recombinant Protein A/G combines four Fc-binding domains from Protein A and two from Protein G, enabling strong affinity for all human IgG subclasses and a wide range of mammalian IgG. -
Universal IgG Affinity Resin
Suitable for antibodies from human, mouse, rat, goat, sheep, cow, rabbit, and more—especially when antibody subclass is unknown. -
Stable & Reusable
Prepared using AminoLink™ Coupling Chemistry for covalent, charge-free immobilization, offering excellent resin stability and reusability over multiple cycles. -
Minimal Nonspecific Binding
Inert matrix and leach-resistant bonds reduce background and improve purification purity.
Technical Specifications
| Parameter | Value |
|---|---|
| Resin Matrix | Crosslinked 6% CL-6B agarose |
| Ligand | Recombinant Protein A/G |
| Binding Capacity | >50 mg human IgG/mL resin |
| Bead Size | 45–165 μm |
| pH Stability | 2–14 (short term), 3–13 (long term) |
| Exclusion Limit | 10,000–4,000,000 Da |
| Max. Flow Rate | ~1 mL/min (1 cm column) |
| Max. Pressure Tolerance | <25 psi (1.5 bar) |
| Format | Bottle (2 mL or 20 mL) |
| Storage Conditions | 4°C (do not freeze) |
Applications
- Purification of polyclonal and monoclonal antibodies from complex samples (e.g., serum, ascites fluid, culture supernatants).
- Ideal for labs processing diverse or uncharacterized antibody subclasses.
- Immunoprecipitation and immunoaffinity workflows requiring high yield and specificity.
How It Works
Pierce Protein A/G Plus Agarose employs a recombinant fusion ligand that captures immunoglobulin G (IgG) via the Fc region. The resin is compatible with neutral pH buffers and offers improved retention of antibody activity due to its mild binding and elution conditions. Its enhanced capacity allows fewer purification cycles, saving time and resources.
Related Products
- Pierce™ Protein A/G Agarose – Standard-capacity option for routine purification
- Pierce™ Protein A or G Plus Agarose – Choose based on species/subclass specificity
- Gentle Ag/Ab Binding and Elution Buffers – For non-denaturing antibody elution
Thermo Scientific™ Pierce™ Protein A/G Plus Agarose provides unmatched flexibility and yield in antibody purification. Its broad reactivity, exceptional binding capacity, and reusable performance make it the go-to choice for high-throughput and sensitive immunopurification tasks.
- Pack Size: 20 mL 2 mL