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Specifications:
| Application | Automated Nucleic Acid Purification | Warranty | 1 Year |
| Storage Temperature | Room Temperature | ||
| Product Type | Sample Prep | ||
| Product Brand | Thermo Fisher Scientific™ | ||
| Product Grade | Molecular Biology | ||
The Thermo Scientific™ KingFisher™ Duo Prime Purification System is a compact, benchtop magnetic particle processor ideal for low-to-medium throughput nucleic acid, protein, and cell purification workflows. Designed to deliver high-quality, reproducible results with minimal hands-on time, the Duo Prime is perfect for labs performing routine or customized extraction protocols from a wide variety of sample types.
Its flexible design accommodates volumes up to 5 mL, supports both DNA/RNA isolation and proteomics, and features automated magnetic bead technology for efficient purification. Additional upgrades such as a built-in UV decontamination lamp and optional barcode reader enhance contamination control and sample traceability.
✅ Key Features & Benefits
🧲 Automated Magnetic Bead Technology
- Utilizes Thermo Fisher’s proven magnetic particle processing, ensuring ≥95% particle collection efficiency.
- Delivers high recovery rates of nucleic acids, proteins, or cells across a wide range of input types.
🔄 Flexible Throughput & Sample Types
- Up to 12 samples per run (12-pin head) or 6 samples (6-pin head).
- Suitable for processing body fluids, blood, bacteria, cell cultures, tissues, and plants.
- Supports elution volumes of 30–130 µL and processing volumes up to 5 mL.
💡 Smart Lab Features
- 8W UV lamp enables easy decontamination between runs (exposure up to 16 hrs).
- Optional barcode reader for seamless sample tracking and traceability.
🧠 Advanced Protocol Control with BindIt™ Software
- Create, modify, and save up to 200 protocols.
- Import methods via PC or USB stick.
- Automatic run log file saving for compliance and traceability.
🌡️ Precision Heating & Cooling
- Heating range: 10°C to 75°C (plate row A).
- Cooling range: 4°C to 75°C (elution strip).
- Facilitates optimal elution and lysis conditions.
⚙️ Specifications
| Feature | Details |
|---|---|
| Catalog Number | 5400110 |
| Sample Throughput | Up to 12 samples (12-pin head), up to 6 (6-pin) |
| Processing Volume | 50 µL–1,000 µL (96 DW); up to 5 mL (24 DW) |
| Elution Volume | 30–130 µL |
| Magnet Head | 12-pin or 6-pin interchangeable |
| Collection Efficiency | ≥95% |
| UV Lamp | 8W, programmable exposure up to 16 hrs |
| Display | Color LCD screen |
| Protocol Storage | ~200 protocols |
| Port Options | USB |
| Software | Thermo Scientific™ BindIt™ |
| Plate Compatibility | Microtiter 96 DW, KingFisher 24 DW |
| Heating Range | 10–75°C (plate); Cooling 4–75°C (elution strip) |
| Dimensions (W × D × H) | 40 × 46 × 34 cm (15.7 × 18.1 × 13.4 in.) |
| Weight | 17 kg (37.5 lbs) |
| Languages Supported | English, French, Spanish, German, Chinese, Japanese, Russian, Portuguese |
🧪 Recommended Applications
-
DNA and RNA purification from:
- Blood, plasma, serum, buccal swabs, FFPE tissue, plants, etc.
- Protein purification and enrichment (e.g., immunoprecipitation)
- Cell isolation and pathogen enrichment
- Veterinary diagnostics
- Forensic sample processing
- Custom assay development and pilot studies
🧬 Use Case Examples
🔬 Academic and R&D Labs
- Optimize protocols with easy-to-edit BindIt software.
- Ideal for labs running limited batches or performing pilot testing.
🧫 Clinical Research & Forensics
- Use UV decontamination to avoid cross-contamination in sensitive applications.
- Perfect for low-volume high-value samples (e.g., forensic samples).
🧪 Biotech & Pharma Labs
- Suitable for biomarker discovery, proteomics, and gene expression workflows.
- Compact design fits easily into molecular biology benches.
🔍 Comparison: KingFisher Duo Prime vs. KingFisher Flex
| Feature | KingFisher Duo Prime | KingFisher Flex |
|---|---|---|
| Throughput | Up to 12 samples | Up to 96 samples |
| Sample Volume | 50 µL – 5 mL | 50 µL – 5 mL |
| Magnet Heads | 6- or 12-pin | 24- or 96-pin (interchangeable) |
| UV Decontamination | Yes (8W lamp) | No |
| Software | BindIt (PC or USB) | BindIt (PC or USB) |
| Heating/Cooling | Yes (plate & elution) | Heating only (up to 115°C) |
| Plate Compatibility | 96 DW, 24 DW | 96 DW, 24 DW, PCR plates |
| Protocol Storage | 200 protocols | 200+ protocols |
| Best for | Low-to-mid throughput, R&D, method development | High-throughput labs, diagnostics, NGS cores |
✅ Why Choose the KingFisher™ Duo Prime?
- Compact and budget-friendly system for labs with limited throughput needs
- Ideal for pilot testing, R&D, teaching labs, and sensitive assays
- Built-in UV lamp for contamination-free operation
- Easy-to-use software with full protocol customization
- Supports a wide range of magnetic bead-based kits and chemistries