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Invitrogen™ MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit, compatible with Tempus™ Blood RNA Tubes, 96 Preps
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Specifications:
| Application | RNA Extraction | ||
| Storage Temperature | -20°C, 2-8°C | ||
| Product Type | Sample Prep | Forms | Kit with Various components |
| Product Brand | Thermo Fisher Scientific™, Invitrogen | ||
| Product Grade | Molecular Biology | ||
The Invitrogen™ MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit provides a reliable solution for purifying total RNA (including miRNA) from blood samples collected in Tempus™ Blood RNA Tubes. Built on proven magnetic bead–based technology, this kit delivers high-quality RNA suitable for a broad range of downstream applications, including RT-PCR, qPCR, cDNA library construction, microarray analysis, NGS, and hybridization assays.
A key feature of this kit is the inclusion of a TURBO DNase™ step, which effectively removes contaminating genomic DNA, ensuring RNA of the highest purity with no inhibitors for sensitive applications.
Key Features
- Magnetic Bead Technology – Eliminates the need for spin columns, enabling a simplified workflow.
- Flexible Formats – Compatible with microfuge tubes, manual 96-well plates, or automated platforms, including KingFisher™ Duo Prime and KingFisher™ Flex.
- High-Quality RNA – RIN ≥ 7; A260/280 ratio of 1.8–2.2; genomic DNA <1.56%.
- Total RNA Recovery – 3–25 µg from a single Tempus™ Blood RNA Tube.
- miRNA Recovery – Proven compatibility with TaqMan™ assays for miRNA detection.
- Scalable Throughput – Adaptable for low-, medium-, and high-throughput needs.
- Rapid Processing – Up to 12 tubes processed manually in ~2 hours.
Applications
- Gene expression profiling via RT-PCR and qPCR.
- Transcriptome analysis including NGS and microarrays.
- miRNA research using blood samples.
- Nuclease protection assays and northern blotting.
- In vitro transcription and translation workflows.
Technical Specifications
| Parameter | Details |
|---|---|
| Product Type | RNA Isolation Kit |
| Catalog Number | 4451893 |
| Quantity | 96 preps |
| Sample Input | 3 mL whole blood in Tempus™ Blood RNA Tubes |
| Yield | 3–25 µg RNA |
| RNA Quality | RIN ≥ 7; A260/280 = 1.8–2.2 |
| Elution Volume | 20–80 µL |
| Format | Magnetic bead–based |
| Throughput | High-throughput compatible |
| Purification Time | <2 hours for automated protocol |
| Shipping Condition | Box 1: Room temp; Box 2: Cold gel |
| Storage | Buffers at RT; Pre-digestion wash and RNA beads at 4 °C; DNase and proteinase at –20 °C |
| Unit Size | Each kit (96 preps) |
Kit Contents
Box 1
- Tempus Resuspension Solution
- Binding Solution (conc.)
- Wash Solutions 1 & 2 (conc.)
- Elution Buffer
- Processing Plate
- Tempus Pre-Digestion Wash
- RNA Binding Beads
- Tempus 1X PBS
Box 2
- Tempus Proteinase
- TURBO DNase
Unique Advantages
- Compatible with the entire Invitrogen blood-to-RNA workflow.
- Efficiently recovers both total RNA and miRNA from stabilized blood.
- Scales seamlessly from manual to automated high-throughput.
- Reduces handling complexity by using magnetic bead separation instead of spin columns.
The Invitrogen™ MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit offers a flexible, scalable, and efficient solution for isolating high-quality total RNA (including miRNA) from Tempus™ Blood RNA Tubes. Delivering RNA with RIN ≥ 7 and minimal genomic DNA contamination, it ensures reproducible results across a range of throughput formats—from manual processing to automation. Ideal for gene expression profiling, miRNA studies, and high-throughput transcriptomic research.