Mabtech ELISpot™ conjugate: Streptavidin‑ALP, 1 mL

Mabtech ELISpot™ Conjugate: Streptavidin-ALP is an enzyme conjugate used in ELISpot assays for the detection of biotinylated antibodies and biotinylated antigens. This Streptavidin-ALP conjugate is specifically optimized for use in ELISpot assays, offering excellent biotin affinity and high residual enzyme activity to ensure efficient detection of target molecules in your experiments. It is not for diagnostic use, and is intended solely for research applications.

This conjugate binds biotinylated components, enabling colorimetric detection through alkaline phosphatase (ALP) activity. The conjugate is particularly useful in immune cell detection and cytokine assays by providing high sensitivity and precision in spotting antigen-specific cells or cytokine-producing cells.

Key Features:

1. Optimized for ELISpot:
   • The Streptavidin-ALP conjugate is specifically designed for ELISpot assays, a popular method for quantifying cytokine-producing cells.
2. High Residual Enzyme Activity:
   • Ensures high sensitivity and accurate detection in ELISpot and other biotinylated antibody-based assays.
3. Strong Biotin Affinity:
   • Offers high biotin-binding capacity, which ensures efficient detection of biotinylated reagents.
4. Formulation:
   • Supplied in 0.1 M Tris buffer with 1% BSA and 0.002% Kathon CG, ensuring stability and compatibility with downstream applications.
5. Recommended Dilution:
   • Dilution is recommended at 1:1000 for general applications, though this may vary depending on the assay conditions.
6. Convenient Shipping and Storage:
   • Shipped at ambient temperature and should be stored at 4-8°C upon receipt for optimal performance. The product has a shelf life of 18 months from the date of receipt.

Specifications:

Applications:

1. ELISpot Assays:
   • Used to detect biotinylated antibodies and biotinylated antigens in immune cell assays by providing a colorimetric readout of cytokine-producing cells or antigen-specific responses.
2. Cytokine Profiling:
   • Facilitates the identification and quantification of specific cytokine-producing cells, making it invaluable for immunology and infection studies.
3. Protein Detection:
   • Ideal for use in protein assays and applications requiring biotin detection such as ELISA and Western blot.
4. Cell-based Assays:
   • Useful for cell-based assays that require specific detection of biomolecules, antigens, or protein interactions.

Related Products:

• Mabtech ELISpot™ Detection Kit
• Mabtech Anti-Human IFN-γ mAb (1-D1K)
• Mabtech Streptavidin-ALP conjugates
• Mabtech Cytokine Assay Kits

The Mabtech ELISpot™ Conjugate: Streptavidin-ALP is a high-performance, reliable tool for biotin detection in immunoassays. It is specially optimized for ELISpot assays to detect cytokine-producing cells and other biotinylated targets. This conjugate’s high enzyme activity, biotin affinity, and stability make it an indispensable reagent for immunology research, protein quantification, and cellular studies.