Sigma-Aldrich™ MultiScreen™ 96-Well Assay Plates, Clear, for ELISPOT Assays; pore size 0.45 μm, sterile

The Sigma-Aldrich™ MultiScreen™ 96-Well Assay Plates are designed specifically for ELISPOT assays and are equipped with hydrophobic PVDF or MCE membranes. These plates are ideal for applications requiring the detection of secreted cytokines from individual cells in a sample, making them a reliable tool for cell-based assays such as immune monitoring and protein secretion analysis. The 0.45 μm pore size ensures efficient filtration and captures relevant biological data in applications ranging from stem cell research to immunology.

Key Features:

1. Hydrophobic PVDF Membrane:
   • Hydrophobic PVDF (Polyvinylidene Fluoride) membranes are optimized for protein and cell binding in ELISPOT applications, ensuring high sensitivity and specificity for cytokine detection.
2. 96-Well Format:
   • The 96-well plate design is a standard format for high-throughput screening (HTS) and can accommodate 50-250 μL sample volume per well.
3. High Binding Surface:
   • Designed with a high binding surface, ideal for capturing a variety of sample types including cardiac stem cells, hematopoietic stem cells, epithelial cells, and mouse or human embryonic stem cells.
4. Clear Plate with Flat Bottom Wells:
   • The clear plate and flat-bottom wells ensure optimal visibility and even distribution of samples.
5. Sterile and Ready to Use:
   • The plate is sterile, ready for use in sensitive experiments where contamination control is crucial.
6. Reagents:
   • The plates are compatible with ELISPOT assays, ideal for detecting cell-based cytokine production and other soluble factors in cell culture supernatants.
7. Wide Range of Applications:
   • This plate is widely used in immunology, cancer research, infectious disease studies, stem cell research, and cell biology for quantifying specific cell functions or biomarkers.

Specifications:

Applications:

1. ELISPOT Assays:
   • MultiScreen™ 96-Well Plates are ideal for performing ELISPOT assays, detecting specific cytokine secretion by individual cells.
2. Stem Cell Research:
   • Suitable for cell-based assays and protein secretion analysis in stem cell research, including cardiac, hematopoietic, and embryonic stem cells.
3. Immunology and Cancer Research:
   • These plates provide an effective platform for studying immune responses and cytokine profiling in cancer research and inflammatory conditions.
4. Cell-Based Assays:
   • Use in high-throughput screening (HTS) for quantifying cytokine production, helping assess immune cell activity and T-cell responses.
5. Infectious Disease Studies:
   • Commonly used for analyzing the immune response in infectious disease models and studying antibody production and antigen responses.

Related Products:

• Thermo Scientific™ ELISPOT Kits
• MultiScreen™ HTS Plates
• MultiScreen™ 384-Well Assay Plates
• Millipore™ Immobilon® PVDF Membrane

The Sigma-Aldrich™ MultiScreen™ 96-Well Assay Plates provide a reliable and versatile tool for ELISPOT assays and other cell-based assays. With a hydrophobic PVDF membrane, high binding capacity, and sterility, these plates are perfect for quantifying cytokine production and analyzing protein secretion in mammalian cells, especially in applications requiring high sensitivity and throughput.