Thermo Scientific™ Pierce™ Amino Acid Standard H, hydrolysates standard, 10 × 1 mL ampules, 2.5 μMol/mL

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    Specifications:
    Application chromatography
    Storage Temperature -20°C
    Product Type Calibration Standard Forms Liquid
    Product Brand Thermo Fisher Scientific™
    Product Grade Analytical grade

    High-Purity Quantitative Standard for HPLC Analysis of Protein Hydrolysates

    Pierce™ Amino Acid Standard H is a quantitative mixture of 18 L-form amino acids, each supplied at 2.5 µmol/mL in 0.1N HCl (except cystine at 1.25 µmol/mL), designed for high-accuracy amino acid composition analysis of peptides and proteins by HPLC. The solution is pre-aliquoted in sealed 1 mL glass ampules to maintain stability and prevent contamination.

    Amino AcidConcentration (µmol/mL)Solvent
    L-Alanine2.50.1N HCl
    L-Arginine2.50.1N HCl
    L-Aspartic Acid2.50.1N HCl
    L-Cystine1.250.1N HCl
    L-Glutamic Acid2.50.1N HCl
    Glycine2.50.1N HCl
    L-Histidine2.50.1N HCl
    L-Isoleucine2.50.1N HCl
    L-Leucine2.50.1N HCl
    L-Lysine·HCl2.50.1N HCl
    L-Methionine2.50.1N HCl
    L-Phenylalanine2.50.1N HCl
    L-Proline2.50.1N HCl
    L-Serine2.50.1N HCl
    L-Threonine2.50.1N HCl
    L-Tyrosine2.50.1N HCl
    L-Valine2.50.1N HCl
    Ammonia (NH₄⁺ source)2.50.1N HCl

    This standard is specifically formulated for calibrating amino acid analyzers and assessing hydrolysates derived from proteins or peptides. It is certified by conventional amino acid analysis and is ideal for both qualitative and quantitative workflows.

    Key Features & Benefits

    • Quantitative Calibration Standard – Each of the 18 amino acids is present at a known, verified concentration
    • Ready-to-Use Format – Supplied in sealed 1 mL ampules, eliminating the need for reagent preparation
    • Stable in 0.1N HCl – Long shelf life when frozen; stable between –20°C and 8°C
    • L-Amino Acids Only – Ensures standardization in microbial, biochemical, and chromatography assays
    • Validated by Amino Acid Analysis – Provides traceable and reproducible performance for compositional studies

    🧪 Applications

    • HPLC Calibration of Amino Acid Analyzers
    • Quantitative Analysis of Protein Hydrolysates
    • Peptide and Protein Composition Verification
    • Microbiological Media Testing
    • Standardization of Amino Acid Assays in Biotech and Pharma

    🔧 Technical Specifications

    AttributeDetails
    Quantity10 × 1 mL sealed ampules
    Concentration2.5 µmol/mL for each amino acid (1.25 µmol/mL for cystine)
    FormLiquid
    Solvent0.1N HCl
    Amino Acids Included18 L-form amino acids
    Shelf Life (Unopened)Indefinite (when frozen)
    Shelf Life (After Opening)6 months (store frozen between uses)
    Storage Conditions–20°C to 8°C
    Regulatory UseFor Research Use Only

    📦 Contents

    • Ten (10) 1 mL ampules of amino acid standard solution
    • Supplied in high-quality packaging to ensure sterility and stability
    • Compatible with standard amino acid analyzers and HPLC systems

    ⚠️ Handling & Usage Notes

    • Shake well after thawing to ensure homogeneity
    • Use appropriate safety precautions when handling glass ampules (e.g., ampule breakers)
    • Avoid repeated freeze-thaw cycles

    Thermo Scientific™ Pierce™ Amino Acid Standard H is the trusted reference solution for amino acid compositional analysis in proteomics and quality control labs. Its accurate, stable formulation, and certified concentrations provide researchers with the confidence to perform sensitive, reproducible, and high-precision analyses of protein hydrolysates using HPLC or amino acid analyzers

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