High-Purity Quantitative Standard for HPLC Analysis of Protein Hydrolysates
Pierce™ Amino Acid Standard H is a quantitative mixture of 18 L-form amino acids, each supplied at 2.5 µmol/mL in 0.1N HCl (except cystine at 1.25 µmol/mL), designed for high-accuracy amino acid composition analysis of peptides and proteins by HPLC. The solution is pre-aliquoted in sealed 1 mL glass ampules to maintain stability and prevent contamination.
Amino Acid | Concentration (µmol/mL) | Solvent |
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L-Aspartic Acid | 2.5 | 0.1N HCl |
L-Glutamic Acid | 2.5 | 0.1N HCl |
L-Phenylalanine | 2.5 | 0.1N HCl |
Ammonia (NH₄⁺ source) | 2.5 | 0.1N HCl |
This standard is specifically formulated for calibrating amino acid analyzers and assessing hydrolysates derived from proteins or peptides. It is certified by conventional amino acid analysis and is ideal for both qualitative and quantitative workflows.
✅ Key Features & Benefits
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Quantitative Calibration Standard – Each of the 18 amino acids is present at a known, verified concentration
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Ready-to-Use Format – Supplied in sealed 1 mL ampules, eliminating the need for reagent preparation
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Stable in 0.1N HCl – Long shelf life when frozen; stable between –20°C and 8°C
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L-Amino Acids Only – Ensures standardization in microbial, biochemical, and chromatography assays
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Validated by Amino Acid Analysis – Provides traceable and reproducible performance for compositional studies
🧪 Applications
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HPLC Calibration of Amino Acid Analyzers
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Quantitative Analysis of Protein Hydrolysates
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Peptide and Protein Composition Verification
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Microbiological Media Testing
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Standardization of Amino Acid Assays in Biotech and Pharma
🔧 Technical Specifications
Attribute | Details |
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Quantity | 10 × 1 mL sealed ampules |
Concentration | 2.5 µmol/mL for each amino acid (1.25 µmol/mL for cystine) |
Form | Liquid |
Solvent | 0.1N HCl |
Amino Acids Included | 18 L-form amino acids |
Shelf Life (Unopened) | Indefinite (when frozen) |
Shelf Life (After Opening) | 6 months (store frozen between uses) |
Storage Conditions | –20°C to 8°C |
Regulatory Use | For Research Use Only |
📦 Contents
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Ten (10) 1 mL ampules of amino acid standard solution
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Supplied in high-quality packaging to ensure sterility and stability
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Compatible with standard amino acid analyzers and HPLC systems
⚠️ Handling & Usage Notes
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Shake well after thawing to ensure homogeneity
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Use appropriate safety precautions when handling glass ampules (e.g., ampule breakers)
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Avoid repeated freeze-thaw cycles
Thermo Scientific™ Pierce™ Amino Acid Standard H is the trusted reference solution for amino acid compositional analysis in proteomics and quality control labs. Its accurate, stable formulation, and certified concentrations provide researchers with the confidence to perform sensitive, reproducible, and high-precision analyses of protein hydrolysates using HPLC or amino acid analyzers
https://www.labmartgh.com/shop/product/20088-thermo-scientific-tm-pierce-tm-amino-acid-standard-h-hydrolysates-standard-10-x-1-ml-ampules-2-5-mmol-ml-30235
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