0
Invitrogen™ PureLink™ Pro 96 total RNA Purification Kit, 4 x 96 Preps
Catalog No :
CAS Number :
Brand :
In Stock
Specifications:
| Application | RNA Extraction | ||
| Storage Temperature | Room Temperature | ||
| Product Type | Sample Prep | Forms | Kit with Various components |
| Product Brand | Thermo Fisher Scientific™, Invitrogen | ||
| Product Grade | Molecular Biology | ||
The Invitrogen™ PureLink™ Pro 96 Total RNA Purification Kit provides a robust, high-throughput system for isolating high-quality total RNA from diverse biological sources including bacteria, yeast, plant tissue, mammalian tissue, and cultured cells. Designed for scalability and reproducibility, this kit enables laboratories to isolate RNA with minimal genomic DNA contamination in as little as 25 minutes (after homogenization).
Using silica-based 96-well filter plates, the kit eliminates the need for hazardous organic solvents while ensuring consistent yields across plates. The optimized buffer chemistry and plate design guarantee clean absorbance ratios (A260/A280, A260/A230 ~1.8), indicating RNA free from protein, guanidine, and ethanol carryover.
Key Features
- Broad Sample Compatibility – Processes RNA from mammalian tissue, plant tissue, yeast, bacteria, blood-derived cells, and more.
- High Throughput – 96-well silica plate system compatible with centrifugation, vacuum manifolds, or automated robotic platforms.
- Fast Protocol – Total RNA purified in ~25 minutes (after homogenization).
- Safe & Efficient – No phenol, chloroform, or organic waste handling required.
- Optimized Plate Design – Semi-skirted plates with annular collar nozzles prevent cross-contamination and ethanol carryover.
- High Yield – Up to 33 μg RNA per sample, depending on input.
Applications
- Total RNA preparation for RT-PCR and qPCR.
- Construction of cDNA libraries.
- Microarray analysis and next-generation sequencing (NGS).
- Northern blotting and other hybridization techniques.
- In vitro transcription, translation, and nuclease protection assays.
Technical Specifications
| Parameter | Details |
|---|---|
| Product Type | RNA Purification Kit (96-well) |
| Catalog Number | 12173011A |
| Quantity | 384 preps |
| Isolation Technology | Silica 96-well plate |
| Sample Types | Bacteria, yeast, mammalian cells, plant tissue, animal tissue |
| Sample Input | ≤109 bacterial cells, ≤107 yeast cells, ≤150 mg plant tissue, ≤100 mg animal tissue |
| Yield | Up to 33 μg RNA |
| Elution Volume | 45–170 μL |
| RNA Quality | A260/A280, A260/A230 ~1.8 |
| Test Time | ~25 minutes |
| Prep Scale | Mini-prep scale per well |
| Throughput | Manual or automated high-throughput compatible |
| Shipping Condition | Room temperature |
| Storage | Buffers at RT |
| Unit Size | Each kit (384 preps) |
Kit Contents
- PureLink Pro 96 Lysis Buffer
- Wash Buffers I & II
- RNase-free Water
- 96-well Total RNA Filter Plates
- 96-well Elution Plates
Unique Advantages
- A single kit for RNA purification across diverse organisms.
- Provides scalable, reproducible, and contamination-free RNA for sensitive downstream applications.
- Eliminates hazardous waste and speeds up high-throughput workflows.
- Outperforms competitor kits in both yield and RNA integrity.
The Invitrogen™ PureLink™ Pro 96 Total RNA Purification Kit is a versatile and powerful system for high-throughput RNA isolation. Delivering fast, high-yield, and ultraclean RNA from multiple sample types, it is ideal for laboratories conducting RT-PCR, NGS, microarray, or hybridization assays at scale. Its compatibility with manual and automated platforms ensures reproducibility and flexibility for diverse RNA workflows.