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Specifications:
| Application | Cell culture | ||
| Storage Temperature | Room Temperature | ||
| Product Type | Buffer | Forms | Powder |
| Product Brand | Thermo Fisher Scientific™ | ||
| Product Grade | Molecular Biology | Formula | C₈H₁₈N₂O₄S |
HEPES (N-2-Hydroxyethylpiperazine-N-2-Ethane Sulfonic Acid) is a zwitterionic buffering agent that provides reliable pH stability in cell culture media. It is commonly used for applications requiring precise pH control during extended manipulation outside CO₂ incubators.
Key Features
- Buffering Range: Effective pH range of 6.8–8.2, suitable for most mammalian cell culture systems.
- Concentration: Typically added at 10–25 mM for optimal buffering capacity.
- Chemical Properties:
- Membrane Impermeable: Does not cross cell membranes, ensuring extracellular pH stabilization.
- Biochemically Stable: Minimal interaction with biochemical reactions.
- Low Absorbance: Negligible absorbance in visible and UV light, making it ideal for optical applications.
Specifications
| Attribute | Details |
|---|---|
| Form | Powder |
| Cell Type | Mammalian Cells |
| Shelf Life | 24 months from date of manufacture |
| Storage Conditions | 15–30 °C |
| Shipping Conditions | Ambient Temperature |
| Unit Size | 1 kg |
| pH Range | 6.8–8.2 |
Applications
- Cell Culture:
- Maintains extracellular pH during extended cell manipulation outside CO₂ incubators.
- Supports mammalian cell growth in serum-free and serum-supplemented media.
- Biochemical Assays:
- Provides consistent pH in enzymatic reactions.
- Suitable for experiments requiring optical transparency due to low UV and visible light absorbance.
- Research-Only Use:
- Designed for laboratory research applications. Not for diagnostic or therapeutic use.
Advantages
- Enhanced Buffering Capacity: Extends stability in non-CO₂ environments.
- Chemical and Enzymatic Stability: Ensures minimal degradation over time.
- Low Interference: Does not significantly affect cellular or enzymatic activities.
Storage and Handling
- Storage Temperature: 15–30 °C in a dry environment.
- Shelf Life: 24 months when stored under recommended conditions.
- Shipping Conditions: Delivered at room temperature for convenience.
Safety and Precautions
- Research Use Only: Not intended for diagnostic or clinical applications.
- Handling: Use personal protective equipment and follow standard laboratory safety protocols.
Gibco™ HEPES (Ultra Pure) is a high-quality buffer ideal for supporting mammalian cell culture and biochemical experiments requiring consistent and reliable pH stabilization.
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