Gibco™ HEPES (Ultra Pure)

HEPES (N-2-Hydroxyethylpiperazine-N-2-Ethane Sulfonic Acid) is a zwitterionic buffering agent that provides reliable pH stability in cell culture media. It is commonly used for applications requiring precise pH control during extended manipulation outside CO₂ incubators.

Key Features

• Buffering Range: Effective pH range of 6.8–8.2, suitable for most mammalian cell culture systems.
• Concentration: Typically added at 10–25 mM for optimal buffering capacity.
• Chemical Properties:
  • Membrane Impermeable: Does not cross cell membranes, ensuring extracellular pH stabilization.
  • Biochemically Stable: Minimal interaction with biochemical reactions.
  • Low Absorbance: Negligible absorbance in visible and UV light, making it ideal for optical applications.

Specifications

Applications

1. Cell Culture:
   • Maintains extracellular pH during extended cell manipulation outside CO₂ incubators.
   • Supports mammalian cell growth in serum-free and serum-supplemented media.
2. Biochemical Assays:
   • Provides consistent pH in enzymatic reactions.
   • Suitable for experiments requiring optical transparency due to low UV and visible light absorbance.
3. Research-Only Use:
   • Designed for laboratory research applications. Not for diagnostic or therapeutic use.

Advantages

• Enhanced Buffering Capacity: Extends stability in non-CO₂ environments.
• Chemical and Enzymatic Stability: Ensures minimal degradation over time.
• Low Interference: Does not significantly affect cellular or enzymatic activities.

Storage and Handling

• Storage Temperature: 15–30 °C in a dry environment.
• Shelf Life: 24 months when stored under recommended conditions.
• Shipping Conditions: Delivered at room temperature for convenience.

Safety and Precautions

• Research Use Only: Not intended for diagnostic or clinical applications.
• Handling: Use personal protective equipment and follow standard laboratory safety protocols.

Gibco™ HEPES (Ultra Pure) is a high-quality buffer ideal for supporting mammalian cell culture and biochemical experiments requiring consistent and reliable pH stabilization.