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Gibco™ Hygromycin B (50 mg/mL)
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Water-soluble antibiotic purified from bacterium Streptomyces hydroscopicus
Specifications:
| Application | Cell culture | ||
| Storage Temperature | 2-8°C | ||
| Product Type | Antibiotic | Forms | Liquid |
| Product Brand | Thermo Fisher Scientific™, Gibco | ||
| Product Grade | Molecular Biology | Formula | C₂₀H₃₇N₃O₁₃ |
Sterile, Ready-to-Use Antibiotic for Eukaryotic and Bacterial Selection
Gibco™ Hygromycin B is a water-soluble aminoglycoside antibiotic purified from Streptomyces hydroscopicus and is commonly used for the selection and maintenance of hygromycin-resistant cells. Supplied as a sterile 50 mg/mL liquid solution, it provides convenience and consistency for applications requiring antibiotic pressure in mammalian or insect cell culture systems.
Hygromycin B is effective at inhibiting protein synthesis by interfering with translocation during translation. Resistance is conferred by the hygromycin resistance gene (hyg or hph) from E. coli, making it compatible with dual-selection systems alongside other antibiotics like Geneticin™, Blasticidin S, or Zeocin™.
Key Features and Benefits
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Convenient Ready-to-Use Format
Supplied as a 50 mg/mL sterile liquid, eliminating the need for reconstitution or sterile filtration. -
Broad Spectrum Activity
Inhibits translation in both prokaryotic and eukaryotic cells, supporting versatile use in transfection and transformation workflows. -
Stable Selection Pressure
Recommended working concentration of 200–500 µg/mL for most applications, ideal for stable cell line generation. -
Dual-Selection Compatible
Acts via a different mechanism than Geneticin™, Blasticidin S, or Zeocin™, making it suitable for multi-antibiotic systems. -
Validated for Cell Culture Use
Suitable for use in mammalian and insect cell culture, ensuring reliability in research applications.
Applications
- Stable transfection and selection of mammalian or insect cells
- Antibiotic selection of cells carrying the hyg/hph resistance gene
- Bacterial and eukaryotic gene expression studies
- Dual-selection experiments with non-overlapping antibiotic resistance genes
- Elimination of non-transfected cells in stable line development
Technical Specifications
| Property | Details |
|---|---|
| Product Name | Hygromycin B (50 mg/mL) |
| Catalog Number | 10687010 |
| Formulation | Liquid, sterile-filtered |
| Concentration | 50 mg/mL |
| Recommended Working Range | 200–500 µg/mL |
| Culture Type | Mammalian and Insect Cells |
| Mechanism of Action | Inhibits protein synthesis (translation) |
| Resistance Gene | hyg or hph from E. coli |
| Sterility | Sterile |
| Volume | 20 mL |
| Storage Conditions | 2°C to 8°C |
| Shipping Conditions | Ambient temperature |
| Product Type | Antibiotic |
| Unit Size | Each |
| Intended Use | For Research Use Only. Not for use in diagnostic procedures. |
Gibco™ Hygromycin B (50 mg/mL) offers a convenient and effective solution for antibiotic selection in cell line generation and maintenance. With broad-spectrum activity and compatibility with other selection systems, it is an essential reagent for researchers working in molecular cloning, transgenics, and stable cell line development.