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Thermo Scientific™ Puromycin dihydrochloride, 98%
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Specifications:
| Application | molecular biology | ||
| Storage Temperature | 2-8°C | ||
| Product Type | Antibiotic | Forms | Solid |
| Product Brand | Thermo Fisher Scientific™, thermo Scientific | ||
| Product Grade | Analytical grade | Formula | C₂₂H₂₉N₇O₅·2HCl·nH₂O |
Puromycin dihydrochloride, an aminoglycoside antibiotic derived from Streptomyces alboniger, is widely used in molecular biology for its potent ability to inhibit protein synthesis. Acting as a structural analog of the 3’-end of aminoacyl-tRNA, puromycin causes premature chain termination during translation, making it an essential tool in gene expression analysis, antibiotic selection, and cancer research. This research-use-only reagent, now under the Thermo Scientific Chemicals brand (formerly Alfa Aesar), is supplied at ≥98% purity, ensuring consistency and reliability in sensitive biological assays.
Key Features
High Purity: ≥98% assay by HPLC for consistent results in research applications
Mode of Action: Inhibits protein synthesis by mimicking aminoacyl-tRNA and inducing early chain termination
Broad Biological Activity: Demonstrates anti-neoplastic, anti-protozoal, and anti-microbial properties
Research-Grade Quality: Provided in powder form; clear, colorless when dissolved
Stable Formulation: Provided as the dihydrochloride hydrate salt for maximum solubility and shelf life
Legacy Alfa Aesar Product: Maintains original SKU (J61278.MB) and specifications post brand transition
RUO Certified: Intended for research use only, not for therapeutic or diagnostic applications
Applications
- In Vitro Cell Culture Antibiotic Selection
- Inhibition of Protein Translation in Eukaryotic Cells
- Gene Regulation and Transcriptional Studies
- Screening of Antitumor Activity (e.g., Brain Tumor Models)
- Antimicrobial Testing Against Gram-Positive Microorganisms
- Protozoal and Trypanosomal Inhibition Research
Technical Specifications
| Attribute | Specification |
|---|---|
| CAS Number | 58-58-2 |
| Synonym | Puromycin hydrochloride |
| Molecular Formula | C₂₂H₃₁Cl₂N₇O₅ |
| Molecular Weight | 544.43 g/mol |
| Purity (HPLC) | ≥97.5% |
| Water Content (KF Titration) | ≤12.0% |
| Appearance | White to pale cream powder |
| Solution Test | Clear, colorless |
| IUPAC Name | (2S)-2-amino-N-[(2S,3S,4R,5R)...dihydrochloride |
| Storage | Store tightly sealed as directed; refer to MSDS |
| Regulatory Classification | RUO – Research Use Only |
Puromycin dihydrochloride (98%) is a cornerstone reagent in molecular biology, cellular research, and oncology, delivering robust translational inhibition and selective cytotoxicity. Its high purity, consistent activity, and compatibility with in vitro protocols make it ideal for academic and industrial labs involved in gene expression, antibiotic selection, and cancer biology. With enduring legacy from the Alfa Aesar portfolio, this reagent upholds Thermo Scientific Chemicals’ standards for quality and performance.
- Pack Size: 100mg 500mg 25mg