Invitrogen™ E-Gel™ 1 Kb Plus DNA Ladder, for 0.8–1% E-Gel agarose gels, ready-to-use
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2 x 1 mL E-Gel 1 Kb Plus DNA Ladder
1 mL 1X E-Gel Sample Loading Buffer
Specifications:
| Application | Gel Electrophoresis | ||
| Storage Temperature | -20°C, 2-8°C | ||
| Product Type | DNA Ladders | Forms | Liquid |
| Product Brand | Thermo Fisher Scientific™ | ||
| Product Grade | Molecular Biology | ||
The Invitrogen™ E-Gel™ 1 Kb Plus DNA Ladder is a ready-to-use molecular weight marker designed for precise sizing and approximate quantification of double-stranded DNA (dsDNA) in the range of 100 bp to 15,000 bp. This DNA ladder consists of 18 individual chromatography-purified DNA fragments, with a reference band at 1,500 bp for easy orientation on gels. The ladder is pre-mixed with loading buffer, making it convenient for immediate use in E-Gel agarose gel electrophoresis.
The E-Gel 1 Kb Plus DNA Ladder is specifically formulated to provide sharp, clear bands with uniform intensity, ensuring high-resolution DNA fragment separation. Ideal for use with 0.8–1% E-Gel agarose gels, it is the preferred choice for accurate DNA sizing and quantification in various research, diagnostic, and educational applications.
Key Features & Benefits
✅ Optimized for E-Gel™ Systems
- Specifically formulated for best performance on E-Gel™ agarose gels, ensuring clear and accurate results in DNA fragment sizing.
- Pre-stained with SYBR™ Gold for fluorescence detection, making it compatible with blue light transillumination.
✅ Broad Size Range
- 100 bp to 15,000 bp—ideal for most DNA fragment analyses, including PCR products, restriction digests, and plasmid preparations.
✅ Convenient and Ready-to-Use
- Premixed with 1X E-Gel Sample Loading Buffer, eliminating the need for separate buffer preparation.
- Just load 1 µL of ladder per well for easy and quick sample loading.
✅ Chromatography-Purified DNA Fragments
- High-quality, sharp bands with consistent and reliable performance.
- Each fragment contains an exact amount of DNA, allowing for approximate quantification of DNA samples based on band intensity.
✅ Stable & Reliable
- Stable at room temperature for up to 6 months, ensuring long shelf life for stock solutions.
- For extended storage, keep the ladder at 4°C or –20°C.
✅ Clear Banding and High Sensitivity
- Uniform band intensity allows for easy visual inspection and accurate molecular weight estimation.
- Suitable for DNA and RNA analysis and general laboratory workflows.
Specifications
| Property | Details |
|---|---|
| Catalog Number | 10488099 |
| Concentration | 0.025 µg/µL |
| Gel Type | Agarose |
| Product Type | DNA Ladder |
| Ready to Use | Yes (premixed with loading buffer) |
| Sample Loading Volume | 1 µL per well |
| Shelf Life | 6 months at room temperature or 4°C |
| Size Range | 100–15,000 bp |
| Gel Compatibility | 0.8–1% E-Gel agarose gels |
| Nucleic Acid Structure | Linear DNA |
| Shipping Condition | Room temperature or on dry ice |
| Technology | Chromatography-purified DNA fragments |
| Unit Size | 100 applications (2 x 1 mL) |
| Contents | 2 x 1 mL E-Gel 1 Kb Plus DNA Ladder, 1 mL 1X E-Gel Sample Loading Buffer |
Applications
- PCR Product Analysis: Sizing and verification of PCR fragments.
- Restriction Digest Analysis: Sizing DNA fragments post-digestion.
- Plasmid Verification: Confirming the correct size of plasmids.
- Genotyping: Sizing DNA fragments for genotyping assays.
- DNA Quantification: Approximate quantification using band intensity.
- Educational & Research Use: Ideal for teaching laboratories and general DNA analysis.
Why Choose the Invitrogen™ E-Gel™ 1 Kb Plus DNA Ladder?
✅ Optimized for E-Gel™ Agarose Systems for fast and efficient DNA analysis
✅ Ready-to-use with no additional preparation required—just load and run
✅ High resolution and precise DNA fragment sizing across a broad range of DNA sizes
✅ Stable and easy to store at room temperature for up to 6 months
✅ Pre-stained with SYBR™ Gold for fluorescence detection, ideal for both gel electrophoresis and real-time PCR applications
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