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Applied Biosystems™ HIV-1 Genotyping Kit with Integrase
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Specifications:
| Application | PCR, Sanger Sequencing | ||
| Storage Temperature | -15°C to -25°C | ||
| Product Type | Sequencing Reagents | Forms | Kit with Various components |
| Product Brand | Thermo Fisher Scientific™, Applied Biosystems™ | ||
| Product Grade | Molecular Biology | ||
Comprehensive Sanger Sequencing-Based Genotyping of HIV-1 Protease, Reverse Transcriptase, and Integrase Regions
Catalog Number: A55120 | Quantity: 48 Tests | Format: Complete RT-PCR + Sequencing Kit
Sample Types: HIV-1 RNA from EDTA plasma or dried blood spots (DBS)
Shipping Condition: Dry Ice | Storage: –15°C to –25°C
The Applied Biosystems™ HIV-1 Genotyping Kit with Integrase is a research-use molecular assay designed to detect and characterize drug resistance mutations (DRMs) within the protease (PR), reverse transcriptase (RT), and integrase (IN) regions of the HIV-1 pol gene. Covering codons 6–99 (PR), 1–251 (RT), and 1–288 (IN), the kit enables sequencing-based identification of resistance mutations associated with protease inhibitors (PI), nucleoside and non-nucleoside reverse transcriptase inhibitors (NRTI, NNRTI), and integrase strand transfer inhibitors (INSTI).
Key Features and Benefits
- Comprehensive Coverage – Simultaneous genotyping of PR, RT, and IN regions of the HIV-1 pol gene
- Subtype Coverage – Detects HIV-1 subtypes A, B, C, D, F, G, CRF01_AE, CRF02_AG, and CRF06_cpx
- Gold-Standard Accuracy – Sanger sequencing ensures high sensitivity and reproducibility
- Complete Workflow Solution – RT-PCR, nested PCR, and bidirectional cycle sequencing in a single kit
- Includes Internal Controls – Positive and negative RNA controls included
- Flexible Sample Input – Use RNA extracted from EDTA plasma or dried blood spots (DBS)
- CDC-Licensed Assays – Designed using validated HIV-1 genotyping methods from the U.S. CDC
Kit Components
- RNA Positive Control, 2 x 100 μL
- HIV RNA Negative Control, 2 x 100 μL
- RT-PCR Master Mix, PR/RT, 2 x 1.055 mL
- RT-PCR Master Mix, IN, 2 x 1.055 mL
- Nested-PCR Master Mix, PR/RT, 2 x 1.285 mL
- Nested-PCR Master Mix, IN, 2 x 1.285 mL
- SuperScript III One-Step RT-PCR with Platinum Taq High Fidelity Enzyme, 2 x 54 μL
- AmpliTaq Gold LD DNA Polymerase, 1 x 54 μL
- Sequencing vector, 2 x 80 μL
- HIV Sequencing Mix R12, 2 x 435 μL
- HIV Sequencing Mix R11, 2 x 435 μL
- HIV Sequencing Mix F12, 2 x 435 μL
- HIV Sequencing Mix F11, 2 x 435 μL
- HIV Sequencing Mix R3, 2 x 435 μL
- HIV Sequencing Mix R2, 2 x 435 μL
- HIV Sequencing Mix R1, 2 x 435 μL
- HIV Sequencing Mix F3, 2 x 435 μL
- HIV Sequencing Mix F2, 2 x 435 μL
- HIV Sequencing Mix F1, 2 x 435 μL
Storage Conditions: –15°C to –25°C
Technical Specifications
| Attribute | Details |
|---|---|
| Target Regions | HIV-1 PR, RT, IN (pol gene) |
| Genomic Coverage | Codons 6–99 (PR), 1–251 (RT), 1–288 (IN) |
| Detection Method | Sanger Sequencing |
| Enzymes Included | SuperScript III, AmpliTaq Gold, Taq High Fidelity |
| Format | RT-PCR, Nested PCR, Cycle Sequencing |
| No. of Reactions | 48 |
| Sample Input | RNA from EDTA plasma or dried blood spots |
| Compatible Instruments | Veriti™, GeneAmp™ 9700, ProFlex™, 3130/3500/SeqStudio Flex Genetic Analyzers |
| Intended Use | Research only (RUO) |
Applications
- Genotyping of HIV-1 for antiretroviral drug resistance research
- Identification of mutations associated with PI, NRTI, NNRTI, and INSTI resistance
- HIV molecular epidemiology studies
- Retrospective clinical and surveillance research
- Viral evolution and resistance mechanism analysis
The Applied Biosystems™ HIV-1 Genotyping Kit with Integrase provides a complete, validated solution for the comprehensive genotyping of key resistance-related regions of the HIV-1 genome. Optimized for use with Sanger sequencing, it ensures accuracy and reproducibility across HIV-1 subtypes, supporting global research into drug resistance monitoring and viral evolution.