Thermo Scientific™ Applied Biosystems™ BigDye™ Terminator v3.1 Cycle Sequencing Kit

The BigDye™ Terminator v3.1 Cycle Sequencing Kit is a robust and highly flexible sequencing solution designed for de novo sequencing, resequencing, and finishing applications. Optimized for long read lengths, this kit enhances sequencing performance across a variety of PCR products, plasmids, fosmids, and BAC templates.

✔ Ideal for AT-rich, GC-rich, and GT-rich sequencing applications

✔ Provides longer, high-quality reads with uniform peak heights and optimal signal balance

✔ Superior accuracy in heterozygote detection and mutation analysis

✔ Compatible with multiple genetic analyzers and thermal cyclers

✔ For Research Use Only. Not for diagnostic procedures.

Key Features & Benefits

✔ Optimized for Long Read Lengths – Delivers high-quality sequence reads with enhanced base calling accuracy.

✔ Better Dye Mobility Characteristics – Ensures clearer signal balance and uniform peak heights, improving heterozygote and mutation detection.

✔ Improved Performance in GT-Rich Regions – Reads through challenging DNA sequences with fewer dropouts and artifacts.

✔ Cost-Effective & High Productivity – Maximizes throughput by minimizing sequencing failures and reducing rework.

Applications & Use Cases

1. De Novo Sequencing

• Purpose: Enables sequencing of novel genomes, transcripts, and large genomic regions.
• Use Case: Researchers use BigDye™ v3.1 for high-throughput whole-genome sequencing projects.

2. Resequencing & Comparative Sequencing

• Purpose: Detects mutations, polymorphisms, and variations in known DNA sequences.
• Use Case: Used for SNP genotyping, disease variant analysis, and comparative genetic studies.

3. Mixed-Base Detection & Heterozygote Analysis

• Purpose: Accurately identifies mutations, indels, and heterozygous variants.
• Use Case: Applied in oncology research and genetic mutation screening for detecting germline or somatic mutations.

4. Sequencing AT-Rich, GC-Rich, and GT-Rich Templates

• Purpose: Overcomes challenges in sequencing high GC-content and repetitive regions.
• Use Case: Optimized for difficult-to-sequence genomic regions and highly structured DNA sequences.

5. Long-Read Sequencing of Various Templates

• Purpose: Provides longer read lengths and high-resolution sequencing for large templates.
• Use Case: Suitable for sequencing BAC DNA, Lambda DNA, plasmid DNA (≤15 Kb), fosmids, and PCR amplicons.

Catalog Numbers & Quantities

Product Specifications

Contents & Storage

✔ Kit Includes:

• BigDye™ Terminator v3.1 Ready Reaction Mix
• M13 (-21) Primer
• pREF-BDT™ Control DNA
• 5X Sequencing Buffer (may be stored at 4°C)

✔ Storage Conditions:

• Store at -15°C to -25°C
• Avoid repeated freeze-thaw cycles

Instrument Compatibility & Software Support

✅ Compatible with:

• SeqStudio™ & SeqStudio™ Flex Series Genetic Analyzers
• Applied Biosystems™ 3500, 3500xL, 3730, 3730xl DNA Analyzers
• Thermal Cyclers: GeneAmp™ PCR System 9700, Veriti™ Fast 96-well & 384-well Thermal Cyclers

Ordering Information

Regulatory & Compliance Information

• For Research Use Only (RUO) – Not intended for diagnostic procedures.
• Manufactured under strict quality control standards for high accuracy and reproducibility.

The BigDye™ Terminator v3.1 Cycle Sequencing Kit is an industry-leading sequencing solution offering high accuracy, long read lengths, and enhanced peak uniformity. It is ideal for de novo sequencing, resequencing, and complex DNA templates, making it a preferred choice for genetic research, mutation detection, and high-throughput sequencing applications.