Applied Biosystems™ Sequencing Standard, BigDye™ Terminator v3.1, for 3500/SeqStudio™ Flex, 4 Tubes

The BigDye™ Terminator v3.1 Sequencing Standard Kit is a high-quality reference material designed for spectral calibration, performance verification, and control sequencing runs on Applied Biosystems™ 3500 Series Genetic Analyzers and SeqStudio™ Flex Series Genetic Analyzers. The kit contains four tubes of lyophilized DNA of a known 1200 base pair sequence, ensuring precise, consistent, and reliable calibration for Sanger sequencing applications.

This sequencing standard is essential for maintaining instrument performance and ensuring accurate results in genetic analysis workflows.

✔ For Research Use Only (RUO). Not for diagnostic procedures.

Key Features & Benefits

✔ Optimized for Spectral Calibration & Performance Checks – Ensures high-quality sequencing data by maintaining system accuracy.

✔ Designed for Applied Biosystems 3500 & SeqStudio Flex Series – Specifically validated for optimal compatibility.

✔ High Stability – Lyophilized DNA enhances long-term stability and reliable storage.

✔ Ready-to-Use – Pre-prepared sequencing standard minimizes preparation time and reduces potential handling errors.

✔ Supports Quality Control in Sequencing – Provides a benchmark for instrument performance validation.

Applications & Use Cases

1. Spectral Calibration for Genetic Analyzers

• Purpose: Ensures the proper separation and detection of fluorescent dyes used in Sanger sequencing.
• Use Case: Researchers and lab technicians run the BigDye Terminator v3.1 sequencing standard to maintain spectral accuracy before sequencing unknown samples.

2. Performance Verification & Quality Control

• Purpose: Validates the sequencing accuracy and consistency of the Applied Biosystems™ 3500 and SeqStudio Flex Series Genetic Analyzers.
• Use Case: Before processing critical genetic samples, labs perform a control sequencing run using this standard to confirm optimal instrument performance.

3. Troubleshooting Instrument Performance Issues

• Purpose: Helps identify potential instrument errors, misalignment, or spectral overlap issues.
• Use Case: If a sequencing system generates unexpected results, running the BigDye Terminator v3.1 standard can help pinpoint calibration errors or reagent inconsistencies.

4. Training & Method Development in Sequencing Labs

• Purpose: Serves as a reference control for sequencing protocol optimization and staff training.
• Use Case: New sequencing personnel can use this standard to learn instrument calibration, sample preparation, and data interpretation.

Product Specifications

Contents & Storage

✔ Includes:

• 4 tubes of 1200 base pair lyophilized DNA, prepared with BigDye™ Terminator v3.1
• Each tube is sufficient for one spectral calibration/performance check and/or one control sequencing run

✔ Storage Conditions:

• Store at -15°C to -25°C for long-term stability.
• Keep tubes sealed and protected from moisture.
• Do not freeze-thaw multiple times to maintain DNA integrity.

Instrument Compatibility

✅ For Use With:

• Applied Biosystems™ 3500 Series Genetic Analyzers
• SeqStudio™ Flex Series Genetic Analyzers

Ordering Information

Regulatory Information

• For Research Use Only (RUO). Not for use in diagnostic procedures.
• Manufactured following strict quality control standards to ensure batch-to-batch consistency.

The BigDye™ Terminator v3.1 Sequencing Standard is an essential tool for ensuring the accuracy, reliability, and calibration of Applied Biosystems™ 3500 and SeqStudio™ Flex genetic analyzers. It provides consistent and high-quality sequencing performance for research laboratories conducting Sanger sequencing applications.