Thermo Scientific™ Applied Biosystems™ ExoSAP-IT™ PCR Product Cleanup Reagent

The ExoSAP-IT™ PCR Product Cleanup Reagent is a one-step enzymatic solution designed for the efficient removal of excess primers and unincorporated nucleotides from PCR reactions. This streamlined cleanup method ensures high recovery rates while eliminating the need for column-based purification, reducing sample loss and the risk of cross-contamination.

✔ Removes excess primers and dNTPs in a single step

✔ Prepares PCR products for sequencing and SNP analysis

✔ Up to 100% recovery of PCR amplicons

✔ Minimizes manual handling and reduces contamination risk

✔ Compatible with multiple downstream applications

✔ For Research Use Only. Not for diagnostic procedures.

Key Features & Benefits

✔ Single-Step, Enzymatic Cleanup

• Eliminates unincorporated nucleotides and primers without additional purification steps.
• Reduces sample loss and processing time compared to traditional column-based purification.

✔ Preserves PCR Amplicons

• Provides up to 100% recovery, ensuring maximum DNA yield for sequencing.
• Directly compatible with capillary electrophoresis and other sequencing platforms.

✔ Simple Workflow & Fast Processing

• Requires only one pipetting step, followed by two incubations:
  1. 37°C incubation – Digests primers and dNTPs.
  2. 80°C incubation – Inactivates enzymes before sequencing or SNP analysis.
• No centrifugation, filtration, or further purification required.

✔ Compatible with a Wide Range of Downstream Applications

• DNA sequencing (Sanger sequencing)
• Single nucleotide polymorphism (SNP) analysis
• Genotyping
• Microarray analysis
• Mutation detection

Applications & Use Cases

1. DNA Sequencing Sample Preparation

• Purpose: Ensures that PCR amplicons are free of excess primers and dNTPs before sequencing.
• Use Case: Researchers performing Sanger sequencing use ExoSAP-IT™ to improve read accuracy and signal quality.

2. SNP Genotyping & Mutation Analysis

• Purpose: Prepares DNA for high-resolution SNP and mutation detection assays.
• Use Case: ExoSAP-IT™ is used in genetic disease research and pharmacogenomics studies to ensure accurate SNP calls.

3. PCR Amplicon Cleanup for High-Throughput Studies

• Purpose: Automates the purification process in high-throughput laboratories.
• Use Case: Used in genotyping and genetic screening workflows for efficient and cost-effective PCR purification.

4. Microarray & Hybridization Sample Preparation

• Purpose: Ensures clean amplicons for microarray-based genetic analysis.
• Use Case: Helps researchers studying gene expression and genetic variations by providing pure PCR products for hybridization.

Catalog Numbers & Quantities

Product Specifications

Contents & Storage

✔ Kit Includes:

• ExoSAP-IT™ PCR Cleanup Reagent (100 - 5000 reactions, depending on catalog number)

✔ Storage Conditions:

• Shipped on dry ice
• Store at -20°C
• Avoid repeated freeze-thaw cycles

Ordering Information

Regulatory & Compliance Information

• For Research Use Only (RUO) – Not intended for diagnostic procedures.
• Manufactured under strict quality control for highly reproducible performance.

The ExoSAP-IT™ PCR Product Cleanup Reagent is a gold-standard solution for efficient enzymatic purification of PCR amplicons. With its one-step, two-incubation process, it provides maximum DNA recovery, eliminates excess primers and dNTPs, and ensures high-quality sequencing and SNP analysis results. It is an ideal choice for researchers and labs performing Sanger sequencing, genotyping, and genetic studies.