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Thermo Scientific™ Pierce™ 660nm Protein Assay Kit
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Sufficient For: 300 tube assays or 3000 microplate assays
• Pierce 660 nm Protein Assay Reagent, 450 mL
• Albumin Standard Pre-Diluted Set, 7 x 3.5 mL
Specifications:
| Application | Protein Assays & Analysis | ||
| Storage Temperature | 2-8°C | ||
| Product Type | Molecular Biology Reagent | Forms | Kit with Various components |
| Product Brand | Thermo Fisher Scientific™ | ||
| Product Grade | Molecular Biology | ||
The Pierce™ 660nm Protein Assay Kit provides a fast, simple, and highly compatible colorimetric method for measuring total protein concentration in biological samples. Unlike traditional dye-based assays such as Bradford, the 660nm assay reagent offers superior compatibility with detergents and reducing agents, making it ideal for protein extracts prepared in complex buffers.
This single-reagent assay produces a stable color shift measurable at 660 nm and supports both microplate and test tube formats with minimal sample volume requirements. It is particularly useful in workflows involving protein purification, lysis buffer quantification, and sample normalization prior to SDS-PAGE, Western blotting, or mass spectrometry.
Key Features and Benefits
- Detergent-Compatible – More tolerant of detergents and reducing agents than Coomassie or BCA-based assays
- Ready-to-Use – Single reagent format; no preparation or mixing required
- Flexible Format – Use in test tubes or 96-well microplates
- Minimal Sample Volume – Requires only 10 µL for microplate or 100 µL for test tube assays
- Stable Signal – Produces consistent absorbance response over time
- Optional Detergent Compatibility Enhancer – Extend use to SDS-containing samples using IDCR (Ionic Detergent Compatibility Reagent)
- Room Temperature Storage – No need for cold storage of assay reagent
How It Works
The 660nm assay relies on the binding of a dye-metal complex to positively charged amino acid residues in proteins (e.g., histidine, lysine, arginine). This interaction induces a color change from reddish-brown to green, with a maximum absorbance at 660 nm, directly proportional to protein concentration.
Specifications
| Attribute | Details |
|---|---|
| Detection Method | Colorimetric (660 nm) |
| Assay Type | Protein Quantitation |
| Format | Test Tube or 96-well Microplate |
| Sample Volume Required | 10 µL (microplate), 100 µL (test tube) |
| Compatibility | Detergents, Reducing Agents, SDS with IDCR |
| Quantification Range | Broad linear response across protein concentrations |
| Specificity | Non-target-specific; total protein detection |
| Storage Conditions | Reagent at RT, Standards at 4°C |
| Throughput | 300 tube assays or 3000 microplate assays |
| Unit Size | 450 mL |
| Catalog Number | 22662 |
Kit Contents
- Pierce 660 nm Protein Assay Reagent, 450 mL
- Albumin Standard Pre-Diluted Set, 7 × 3.5 mL vials
Related Accessories
- Pierce Ionic Detergent Compatibility Reagent (IDCR) – For compatibility with Laemmli buffer and SDS
- Pierce™ 660nm Protein Assay Reagent (Refill)
Applications
- Protein quantification from cell lysates and purified fractions
- Sample normalization prior to electrophoresis or Western blotting
- Protein analysis from detergent-rich buffers
- Routine total protein assays in proteomics and biochemistry labs
The Thermo Scientific™ Pierce™ 660nm Protein Assay Kit offers a quick, reliable, and detergent-compatible method for determining total protein concentration. Its one-step, ready-to-use format and broad reagent compatibility make it a practical alternative to Bradford and BCA assays, especially for samples prepared in complex lysis buffers. With robust performance and high throughput capacity, this kit is an essential tool for protein quantification in modern research workflows.