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Thermo Scientific™ Ionic Detergent Compatibility Reagent for Pierce™ 660nm Protein Assay Reagent, 5 x 1g
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Specifications:
| Application | Protein Assays & Analysis | ||
| Storage Temperature | 2-30°C | ||
| Product Type | Molecular Biology Reagent | Forms | Solid |
| Product Brand | Thermo Fisher Scientific™ | ||
| Product Grade | Molecular Biology | ||
The Ionic Detergent Compatibility Reagent (IDCR) is a specialized additive developed for use with the Pierce™ 660nm Protein Assay Reagent (Cat. Nos. 22662 and 22660). This reagent allows the assay to be used with protein samples prepared in SDS-containing buffers, such as Laemmli sample buffer with bromophenol blue, which are typically incompatible with traditional protein quantitation methods.
IDCR is fully soluble and does not affect the assay's sensitivity, enabling reliable total protein detection even in the presence of high concentrations of ionic detergents. It ensures the accuracy and reproducibility of protein concentration measurements in denaturing buffer conditions common in SDS-PAGE sample prep.
Key Features and Benefits
- Expands Assay Compatibility – Enables accurate protein measurement in the presence of SDS and bromophenol blue
- Maintains Sensitivity – Does not interfere with colorimetric response or absorbance at 660 nm
- Easy to Use – Fully dissolves with thorough mixing
- Enhances Workflow Flexibility – Makes the Pierce™ 660nm Assay suitable for a broader range of buffer systems
- Ideal for SDS-PAGE Samples – Supports sample types containing ionic detergents without compromising assay performance
Specifications
| Attribute | Details |
|---|---|
| Product Name | Ionic Detergent Compatibility Reagent (IDCR) |
| Catalog Number | 22663 |
| Unit Size | 5 × 1 g |
| Detection Method | Colorimetric (660 nm) |
| For Use With | Pierce™ 660nm Protein Assay Reagent |
| Compatibility | SDS-containing buffers (e.g., Laemmli buffer) |
| Format | Solid (powder, fully dissolves in solution) |
| Equipment | Microplate Reader, Spectrophotometer |
| Storage Temperature | 2–25°C |
| Application | Total protein quantification in detergent-rich samples |
| Product Line | Pierce™ |
Kit Contents
- 5 × 1 g vials of Ionic Detergent Compatibility Reagent
Related Products
- Pierce™ 660nm Protein Assay Kit (Cat. No. 22662)
- Pierce™ 660nm Protein Assay Reagent (Cat. No. 22660)
The Thermo Scientific™ Ionic Detergent Compatibility Reagent enhances the flexibility and robustness of the Pierce™ 660nm Protein Assay, making it suitable for protein quantitation in samples containing ionic detergents like SDS. It is an essential companion reagent for researchers working with denaturing buffers and ensures accurate, colorimetric detection of total protein in a wide variety of sample conditions.