Gibco™ Trypsin (2.5%), no phenol red, 100mL

Gibco™ Trypsin (2.5%), no phenol red, is a liquid formulation of proteases derived from porcine pancreas. This product is widely used in cell culture for the dissociation of adherent cells, cell passaging, and primary tissue dissociation. Manufactured under cGMP conditions, this sterile-filtered trypsin solution meets stringent quality and traceability standards, ensuring consistent and reliable performance in research applications.

Key Features:

1. High-Quality Protease Mixture:
   • Derived from porcine pancreas and irradiated for safe use in cell culture.
2. Broad Applications:
   • Suitable for cell dissociation, routine passaging, and tissue dissociation in a wide variety of mammalian cells.
3. Phenol Red-Free:
   • Eliminates pH indicator, making it ideal for sensitive applications.
4. Validated Performance:
   • Tested for pH, osmolality, sterility, and dissociation performance, ensuring reliability and consistency.
5. Comprehensive Quality Assurance:
   • Raw materials are verified for endotoxin levels, sterility, and viral inactivation.

Specifications:

Applications:

1. Cell Culture Passaging:
   • Efficiently dissociates adherent cells for subculturing.
2. Primary Tissue Dissociation:
   • Facilitates the isolation of cells from primary tissues for downstream applications.
3. Cell Recovery and Processing:
   • Enables the collection and processing of cells for various research studies.
4. High-Throughput Workflows:
   • Suitable for automated cell culture systems due to consistent performance.

Advantages:

1. Validated Quality:
   • Each batch is rigorously tested for sterility, pH, osmolality, and dissociation performance.
2. Regulatory Support:
   • Provides detailed documentation, including lot traceability and animal origin certificates, for regulatory compliance.
3. cGMP Manufacturing:
   • Produced in ISO 13485-certified facilities, ensuring high standards of quality and supply chain continuity.
4. Phenol Red-Free:
   • Eliminates potential interference in assays that require phenol red-free reagents.
5. Convenient Storage and Shelf Life:
   • Long shelf life of 24 months and stable storage at -5°C to -20°C.

Protocol Overview:

1. Prepare Cells:
   • Wash cells with a balanced salt solution to remove serum, which can inhibit trypsin activity.
2. Apply Trypsin:
   • Add an appropriate volume of Gibco™ Trypsin (2.5%), no phenol red, to cover the cells.
3. Incubate:
   • Incubate at 37°C, monitoring cell detachment under a microscope.
4. Stop the Reaction:
   • Add a medium containing serum or a trypsin inhibitor to neutralize the enzyme.
5. Collect Cells:
   • Transfer cells to a centrifuge tube for further processing or passaging.

Ordering Information:

Storage and Handling:

• Storage Conditions: Store at -5°C to -20°C.
• Shelf Life: 24 months from the date of manufacture.
• Handling: Avoid repeated freeze-thaw cycles to maintain product integrity.

Related Products:

• Gibco™ TrypLE Express (Animal-Origin Free): For animal origin-free cell dissociation.
• Gibco™ DMEM/F-12 Medium: For cell culture and passaging.
• Gibco™ PBS Without Calcium and Magnesium: For washing cells before trypsinization.

The Gibco™ Trypsin (2.5%), No Phenol Red provides a reliable, high-quality solution for cell culture workflows, ensuring optimal cell dissociation while maintaining regulatory compliance and research-grade performance.