Gibco™ DMEM/F-12, powder

Gibco™ DMEM/F-12 (Dulbecco's Modified Eagle Medium: Nutrient Mixture F-12) is a versatile basal medium widely used for the growth of various mammalian cell types. This medium combines the high concentrations of glucose, amino acids, and vitamins from DMEM with the broad spectrum of nutrients from Ham's F-12, making it suitable for numerous cell culture applications.

Key Features

• 1:1 DMEM and F-12 Mix: Combines the benefits of DMEM and Ham's F-12 to support diverse cell types.
• No Added Proteins or Growth Factors: Requires supplementation, typically with 10% Fetal Bovine Serum (FBS).
• Flexible Usage: Suitable for culturing MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts.
• Powder Formulation: Offers convenience for long-term storage, preparation, and customized medium adjustments.

Modifications

With:

• High Glucose
• L-Glutamine
• Phenol Red

Without:

• HEPES
• Sodium Bicarbonate

Applications

• Primary Cell Cultures: Optimal for primary fibroblasts, glial cells, and endothelial cells.
• Cell Lines: Supports HeLa, MDCK, and fibroblast cell lines.
• High Throughput Screening: Ideal for assays requiring a balanced medium for consistency across experiments.
• Research & Development: Frequently used in stem cell research, protein expression studies, and regenerative medicine.

Preparation

• Powder Medium: Requires the addition of sodium bicarbonate, pH adjustment, and filtration before use.
• CO₂ Requirement: Compatible with a sodium bicarbonate buffer system, requiring 5–10% CO₂ for maintaining physiological pH.

Specifications

Available Sizes

Usage Considerations

• Supplements Required: Commonly supplemented with 10% FBS for optimal cell growth.
• Storage and Preparation: Store powder at 2–8°C and prepare fresh medium with sodium bicarbonate before use.
• CO₂ Compatibility: Requires a 5–10% CO₂ incubator to maintain pH.

Recommended Applications

• Basic Research: Protein expression, gene editing, and drug screening.
• Stem Cell Culture: Ideal for supporting embryonic and adult stem cells with appropriate supplements.
• Molecular Biology: Useful in assays such as transfection, cloning, and imaging experiments.

By offering versatility, ease of preparation, and broad application potential, Gibco™ DMEM/F-12 Powder is an excellent choice for researchers working across multiple cell culture platforms.