Gibco™ DMEM/F-12, powder, HEPES, 10L

Gibco™ DMEM/F-12 (Dulbecco's Modified Eagle Medium: Nutrient Mixture F-12) is a widely used basal medium designed to support the growth of a wide variety of mammalian cell types. The addition of HEPES buffer makes this formulation particularly suited for environments where precise pH control is essential, even outside of CO₂ incubators.

Key Features

• 1:1 DMEM and Ham's F-12 Mixture: Combines the nutrient richness of DMEM with the diversity of components in Ham's F-12.
• HEPES Buffer System: Provides improved pH stability, making it ideal for experiments requiring extended handling or non-CO₂ incubators.
• Customizable: Requires supplementation, commonly with 10% Fetal Bovine Serum (FBS) for optimal cell growth.
• Powder Form: Allows for long-term storage and preparation flexibility.

Modifications

With:

• L-glutamine
• HEPES
• Phenol Red

Without:

• Sodium Bicarbonate

Applications

• Primary Cell Cultures: Suitable for culturing MDCK cells, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts.
• High-Precision Studies: Optimal for experiments where environmental pH fluctuations must be minimized.
• Versatile Use: Commonly employed in basic research, molecular biology, protein production, and tissue engineering.

Preparation and Use

• Powder Medium: Requires sodium bicarbonate supplementation, pH adjustment, and filtration during preparation.
• CO₂ Incubation Optional: With the HEPES buffer system, pH stability can be maintained even in non-CO₂ environments.
• Supplementation Required: Typically supplemented with FBS or other growth factors based on specific research needs.

Specifications

Advantages of HEPES Buffer

1. pH Stability: Ideal for open systems or long experiments outside controlled incubators.
2. Flexible Applications: Suitable for a wide range of mammalian cell types and research conditions.
3. Reduced CO₂ Dependence: Useful in laboratories with limited access to CO₂ incubators.

Recommended Applications

• Basic Research: Includes molecular biology, protein expression, and drug screening.
• Precision Studies: Supports experiments that demand stringent environmental control.
• Tissue Engineering: Provides a stable medium for developing tissue constructs and scaffolds.

Usage Recommendations

1. Store powder at 2–8°C, protected from light.
2. Prepare medium fresh with sodium bicarbonate and adjust pH before filtration.
3. Use in CO₂ or non-CO₂ environments depending on experimental requirements.

By offering a robust nutrient profile combined with pH stability, Gibco™ DMEM/F-12 Powder with HEPES ensures reliable and consistent performance across a broad range of applications.