Gibco™ TrypLE™ Express Enzyme (1X), no phenol red

Gibco™ TrypLE™ Express Enzyme (1X), No Phenol Red, is a highly pure, animal origin-free (AOF) recombinant enzyme designed for gentle and efficient dissociation of adherent mammalian cells. It serves as a direct replacement for trypsin, offering superior performance, improved cell viability, and enhanced safety due to its exceptional purity and animal-free composition.

Key Features:

1. Gentle on Cells:
   • Cleaves peptide bonds at the C-terminal sides of lysine and arginine with minimal cell damage, ensuring higher viability.
2. Animal Origin-Free (AOF):
   • Free from animal-derived components, making it suitable for sensitive applications and regulatory requirements.
3. Stable at Room Temperature:
   • Can be stored at 15–30°C for up to 24 months, offering flexibility and ease of handling.
4. Phenol Red-Free:
   • Suitable for assays requiring phenol red-free conditions, reducing interference in experimental workflows.
5. Ready-to-Use:
   • Provided in a 1X concentration, requiring no further dilution or preparation for standard protocols.
6. Self-Inactivating:
   • Can be inactivated by dilution alone, eliminating the need for trypsin inhibitors like FBS.

Specifications:

Applications:

1. Adherent Mammalian Cell Culture:
   • Efficiently dissociates various cell types, including CHO, HEK 293, A549, primary human keratinocytes, and embryonic stem cells.
2. Primary Cell Isolation:
   • Suitable for gentle dissociation of primary tissues into single-cell suspensions.
3. Cell Passaging:
   • Ideal for routine subculturing of adherent cells.
4. High-Sensitivity Assays:
   • Supports workflows that require phenol red-free reagents for minimal interference.
5. Animal-Free Research:
   • Complies with animal origin-free standards, enabling its use in regulated and sensitive experiments.

Advantages:

1. High Purity:
   • Reduces non-specific cleavage and cell damage compared to porcine-derived trypsin.
2. Easy Integration:
   • Directly replaces trypsin in standard protocols without modification.
3. Regulatory Compliance:
   • Manufactured in cGMP-certified facilities under ISO 13485 standards.
4. Convenient Storage:
   • Stable across a wide range of temperatures, reducing storage limitations.
5. Environmentally Friendly:
   • Energy-efficient manufacturing with sustainable packaging.

Protocol Overview:

1. Pre-Warm TrypLE™:
   • Warm TrypLE™ Express to room temperature before use.
2. Wash Cells:
   • Wash adherent cells with PBS or equivalent to remove serum, which can inhibit enzyme activity.
3. Add TrypLE™:
   • Add enough TrypLE™ Express to cover the cell monolayer.
4. Incubate:
   • Incubate at 37°C, monitoring under a microscope until cells detach (2–5 minutes).
5. Neutralize:
   • Dilute TrypLE™ Express with complete growth medium (no trypsin inhibitor required).
6. Centrifuge and Resuspend:
   • Centrifuge cells, discard the supernatant, and resuspend in fresh medium.

Ordering Information:

Storage and Handling:

• Storage Conditions:
  • Store at 15–30°C, 2–8°C, or -5°C to -20°C, protected from light.
• Shelf Life:
  • 24 months from the date of manufacture.

Related Products:

• Gibco™ PBS Without Calcium and Magnesium: For washing cells prior to dissociation.
• Gibco™ DMEM/F-12 Medium: For neutralizing and culturing dissociated cells.
• Gibco™ TrypLE Select: Alternative phenol red-free enzyme for specific applications.

The Gibco™ TrypLE™ Express Enzyme (1X), No Phenol Red, is a versatile, high-performance dissociation reagent designed to enhance cell viability and experimental reproducibility. Its animal origin-free formulation, exceptional purity, and ease of use make it a superior choice for a wide range of cell culture applications.