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Specifications:
| Application | Cell culture | ||
| Storage Temperature | -20°C | ||
| Product Type | Antibiotic | ||
| Product Brand | Thermo Fisher Scientific™, Gibco | ||
| Product Grade | Molecular Biology | Formula | C₅₅H₈₃N₁₉O₂₁S₂Cu |
High-Potency Glycopeptide Antibiotic for Stable Selection in Mammalian, Insect, Yeast, Bacterial, and Plant Cells
Gibco™ Zeocin™ Selection Reagent is a sterile-filtered liquid formulation of phleomycin D1, a glycopeptide antibiotic produced by Streptomyces verticillus. Zeocin™ belongs to the bleomycin/phleomycin family and exerts its cytotoxic activity by binding to and cleaving DNA, which leads to rapid cell death in non-resistant cells. This mechanism makes it highly effective for selecting genetically modified cells that express a Zeocin resistance gene (Sh ble), commonly included in many expression and transfection vectors.
Supplied as a 100 mg/mL solution in sterile water, Zeocin™ is suitable for use in a wide range of cell types and organisms, including mammalian cells, insect cells, yeast, bacteria, and plant cultures.
Key Features and Benefits
-
High-Activity Glycopeptide Antibiotic
Contains phleomycin D1, which causes double-stranded DNA breaks, leading to rapid cytotoxicity in sensitive cells. -
Broad Compatibility
Effective for selection in mammalian, insect, yeast, bacterial, and plant systems—ideal for multi-platform workflows. -
Rapid and Reliable Selection
Enables efficient elimination of non-transfected or non-transformed cells, ensuring only stably modified cells survive. -
Convenient Ready-to-Use Format
Supplied as a 100 mg/mL sterile-filtered solution, eliminating the need for reconstitution or filtration. -
Stable and Easy to Store
Ships on ice and should be stored at –5°C to –20°C, with a long shelf life under proper conditions.
Applications
- Selection of mammalian and insect cells carrying the Sh ble resistance gene
- Generation of stable cell lines following plasmid transfection
- Antibiotic selection in yeast and E. coli transformation workflows
- Transgenic plant selection using Zeocin-resistant vectors
- Dual-antibiotic selection with other agents like Hygromycin B or Blasticidin
Recommended Working Concentrations
| Organism / System | Working Concentration (µg/mL) |
|---|---|
| Mammalian cells | 50–400 µg/mL |
| Insect cells | 50–200 µg/mL |
| Yeast cells | 75–100 µg/mL |
| Bacterial cells | 25–50 µg/mL |
| Plant cells | 50–100 µg/mL |
A kill curve is recommended to determine the optimal concentration for your specific cell type.
Technical Specifications
| Property | Details |
|---|---|
| Product Name | Zeocin™ Selection Reagent |
| Catalog Number | R25005 |
| Active Compound | Phleomycin D1 |
| Concentration | 100 mg/mL |
| Formulation | Sterile-filtered aqueous solution |
| Format | Liquid |
| Volume | 50 mL |
| Sterility | Sterile-filtered |
| Storage Conditions | –5°C to –20°C (protect from light) |
| Shipping Conditions | Ships on ice |
| Shelf Life | Long-term stable under proper conditions |
| Culture Types Supported | Mammalian, Insect, Bacterial, Yeast, Plant |
| For Use With Applications | Stable transfection, selection of transformed cells |
| Product Type | Antibiotic |
| Product Line | Zeocin™ (Gibco™, Thermo Fisher Scientific) |
| Unit Size | Each (50 mL) |
| Intended Use | For Research Use Only. Not for use in diagnostic procedures. |
Gibco™ Zeocin™ Selection Reagent is a powerful and versatile antibiotic that offers researchers a dependable method for eliminating non-transfected cells across a wide variety of host organisms. Whether you're generating mammalian stable lines, performing yeast transformations, or engineering transgenic plants, Zeocin™ provides consistent and potent selection pressure backed by Thermo Fisher’s renowned quality standards.
- Pack Size: 50 mL 8 x 1.25 mL